Macrophages are specialized cells that rapidly recognize, engulf, and destroy pathogens or apoptotic cells. One of the fundamental features of macrophages is their high plasticity, which allows them to respond to stimuli from the complex tissue microenvironment,
by changing rapidly their functional profile through polarization. Macrophages reveal specific phenotypes and functions in response to different triggers. Depending on the types of stimuli, they can polarize to M1 (pro-inflammatory) or M2 (anti-inflammatory)
macrophages.
Creative Biolabs has developed a range of high-quality macrophage cell lines from different species and sources, including M0, M1, and M2 macrophages.
When commercially available off-the-shelf (COTS) products are not enough, trust Creative Biolabs to develop a solution that will provide the best results you require. Our team will work with you to deliver the perfect solution you expect. Please do not
hesitate to contact us and see how we can help you with your project.
Product List Related ServicesPublications Applications Scientific Resources Q & A Customer Reviews
Related Services
In addition to a range of useful products to assist our customers in macrophage therapeutic development, cell therapy research, and disease modeling, Creative Biolabs combines deep industry-leading expertise with an innovative platform to provide
a full range of high-quality services to support the development of macrophage therapeutics.
Our scientists offer a comprehensive portfolio of services including macrophage isolation and culture services, macrophage polarization assays, macrophage polarization phenotype identification and characterization services, macrophage reprogramming services,
macrophage model development services, and much more. You can browse below and click for details.
Creative Biolabs has built a highly experienced team of scientists and quality staff that have a long history in macrophage isolation and culture. Human monocyte, human alveolar, murine peritoneal cavity, murine bone marrow, murine lung, and murine adipose
tissues are available for macrophage isolation and culture.
Creative Biolabs has developed different types of macrophage models to provide a one-stop solution for a wide range of studies, including immune response, drug development, and disease mechanisms.
Creative Biolabs provides overall solutions for macrophage reprogramming, including employing gene regulatory networks, nanoparticles, microRNA and in vivo Lentivirus-based reprogramming.
Efficient strategies
Comprehensive powerful platform
Address the scientific and technical challenges in macrophage reprogramming
Publications
Jade D. Bailey et al. described a novel and optimized protocol for culturing bone marrow-derived macrophages (BMDMs). They used classically activated macrophages isolated from WT- and NO-deficient mice to develop a standardized
culture method that maximizes cell health and viability. The murine macrophage culture protocol they defined standardizes conditions for cell differentiation, culture, and inflammatory stimulation.
The schematic below depicts well-established LCM-dependent BMDM cultures and the latest optimized BMDM culture method using a combination of MCSF and GMCSF.
Fig. 1 Optimisation of BMDM culture in vitro.1
Applications
Our offerings are designed to facilitate a wide array of applications in immunology, cancer research, drug development, and regenerative medicine. Below are the detailed applications of our macrophage products:
Immunological Research
Our macrophage products can be used to study the roles of macrophages in innate and adaptive immunity, including cytokine production, antigen presentation, and phagocytosis.
Researchers can utilize macrophages to explore mechanisms of inflammation and identify potential therapeutic targets for inflammatory diseases.
Cancer Research
Our products enable investigations into how macrophages interact with tumor cells, contributing to tumor progression and metastasis.
Products can be used to study the polarization of macrophages into M1 or M2 phenotypes and their implications for cancer therapies.
Innovators can explore macrophage-targeting strategies for cancer immunotherapies, assessing their efficacy and safety through in vitro and in vivo experiments.
Infectious Disease Research
Use macrophages to study how various pathogens (bacteria, viruses, fungi) evade immune responses and establish infections.
Assess macrophage responses to vaccine candidates, helping to optimize formulations for enhanced immunogenicity.
Neuroimmunology Research
Explore the role of macrophages in central nervous system disorders, such as multiple sclerosis and Alzheimer’s disease.
Investigate how macrophages contribute to the integrity and function of the blood-brain barrier in health and disease.
Drug Development and Screening
Macrophages are essential for evaluating the safety profiles of new pharmaceutical compounds, especially in terms of immune response and inflammation.
By profiling macrophage responses to drug candidates, researchers can identify biomarkers predictive of therapeutic responses.
Q: What specific macrophage products are available for research use?
A: We offer a wide range of macrophage-related products, including isolated primary macrophages, immortalized macrophage cell lines, macrophage culture media, and macrophage
activation or polarization kits. Our collection also includes products for specific macrophage subtypes, such as M1 and M2 macrophages, as well as reagents for studying macrophage differentiation, cytokine release, and phagocytic activity.
Q: Can your macrophage products be used for high-throughput screening applications?
A: Absolutely! We provide macrophage products and reagents optimized for high-throughput screening applications, including cell-based assays for drug
screening or toxicity studies. Our macrophage cell lines are easy to culture and maintain for large-scale experiments, and we also offer macrophage-based reporter systems and phagocytosis assay kits that are compatible with automated platforms. These
products come with user-friendly protocols that ensure efficient data generation in high-throughput environments.
Q: Do you offer custom macrophage isolation services for specific tissue types?
A: Yes, we offer custom macrophage isolation services from various tissue sources such as lung, liver, spleen, and bone marrow. Our custom services can be
tailored to your specific research requirements, whether you need macrophages from diseased tissue, specialized immune environments, or certain donor profiles. We ensure high purity and viability through optimized isolation protocols and provide complete
documentation to verify the quality of the isolated cells.
Q: Can your macrophage products be used in co-culture systems with other immune cells?
A: Yes, our macrophage products are compatible with co-culture systems, allowing you to study complex immune interactions between macrophages and other
immune cells such as T cells, B cells, or dendritic cells. Our products are available in formats that support co-culture experiments, and we provide specialized media and reagents that promote macrophage survival and function within these systems.
Additionally, our technical team can offer guidance on co-culture setup and experimental design.
Customer Reviews
Exceptional purity and viability, but a few minor issues
The macrophage products arrived in excellent condition with high viability. I used them for in vitro experiments, and they responded well to the stimuli I applied. However, I wish there was more detailed guidance on how to handle them post-thawing, as I had some minor difficulties there.
United States
08-22-2024
Good for co-culture systems
We used these macrophage products in co-culture systems with cancer cells. They integrated well into our experimental setup and showed expected phenotypic changes during interaction with the tumor cells. The differentiation markers were expressed as anticipated, adding credibility to our findings.
United States
08-25-2024
Reproducible results, but occasional inconsistencies
For the most part, these macrophages have provided reliable, reproducible results. However, I did notice slight variations between different batches in terms of their initial viability. Most of the time they were consistent, but I had one instance where they didn’t behave as uniformly as previous batches. It's not a huge issue, but something to keep in mind if you're working on particularly sensitive experiments.
United States
08-29-2024
Robust and long-lasting in culture
They stayed viable in culture longer than I anticipated. That said, after a certain point, I noticed a gradual decline in their responsiveness to stimuli. This is somewhat expected. Overall, though, they did maintain functionality for a reasonable amount of time, making them a good option for most long-term studies.
United States
08-29-2024
Reference
Bailey, Jade D., et al. "Isolation and culture of murine bone marrow-derived macrophages for nitric oxide and redox biology." Nitric Oxide 100 (2020): 17-29, used under [CC BY 4.0](https://creativecommons.org/licenses/by/4.0/).
Fig. 1 Optimisation of BMDM culture in vitro.1
Datasheet