The Antigen-Presenting Capacity Is a Crucial Target for Macrophage Therapeutics

Macrophages are the primary antigen-presenting cells (APCs) found in inflammatory sites, specialized in efficiently clearing necrotic and apoptotic material. However, the antigen-presenting capacity of macrophages in facilitating the immune response has not been well assessed. Based on the thymidine incorporation assay, Creative Biolabs has developed an antigen-presenting capacity assessment service to characterize macrophages. As a gold standard, thymidine incorporation assay has great application potential to study the antigen-presenting capacity of macrophages and thereby promote associated drug discovery.

Fig.1 Antigen cross-presentation potential of macrophage subtypes.¹

Antigen-Presenting Capacity Assessment Service by Thymidine Incorporation Assay at Creative Biolabs

Antigen-presenting capacity assessment service by thymidine incorporation assay from Creative Biolabs is based on a radioactive assay that incorporates the radioactive [3H] thymidine into newly synthesized DNA. We evaluate the macrophages' ability to present antigens by measuring the levels of radioactivity in the antigens that were radiolabeled.

Furthermore, we provide a service to isolate macrophages from human or murine tissues and culture them. Moreover, we also provide custom solutions to aid in the development of associated drugs or therapies targeting the antigen-presenting capacity of macrophages. At Creative Biolabs, we not only possess complete and professionally certified equipment to ensure this service but also equip a professional and experienced research team for each project. Together with them, we are confident in presenting desirable results for customers around the world at a short turnaround.

Fig.2 Schematic diagram of antigen-presenting capacity assessment service by thymidine incorporation assay.²

In addition, we provide full-around customized services involving both macrophage primary cells and macrophage cell lines in accordance with customers' diverse needs.

• Macrophage primary cells: bone marrow, blood, spleen, etc.
• Macrophage cell lines: J774A.1, RAW264.7, P388D1, U937, etc.

Features of Our Antigen-Presenting Capacity Assessment Service by Thymidine Incorporation Assay

• Gold standard thymidine incorporation assay.
• Macrophage cells from various sources.
• Less sample consumption and higher reproducibility.
• Easy-to-operate procedures.
• Professional equipment configuration.
• Experience research teams equipped for each project.
• One-stop comprehensive customized services.

Potential Applications

• Helps to deeply understand the antigen presentation mechanism of macrophages, and thereby aid in studying the function and regulation of the immune system.
• Helps to identify and screen macrophage subpopulations with efficient antigen presentation capabilities.
• Helps to evaluate the effect of drugs or therapies on macrophage antigen presentation ability, providing strong support for the development of macrophage-targeted drugs, immunotherapy, etc.

With our solid professional knowledge and abundant project experience, Creative Biolabs is constantly developing useful technical services to support customers' macrophage projects. We encourage you to get in touch with us for more details about our antigen-presenting capacity assessment service by thymidine incorporation assay and place an order.

References

1. Muntjewerff, Elke M., et al. "Antigen cross-presentation by macrophages." Frontiers in Immunology 11 (2020): 1276.
2. Ganesan, Nirosha, et al. "Methods to Assess Proliferation of Stimulated Human Lymphocytes In Vitro: A Narrative Review." Cells 12.3 (2023): 386.