Macrophage-Mesenchymal Stromal Cell Interaction Analysis Service

Mesenchymal stromal cells (MSCs) have a significant interaction with macrophages, marking them as potential cell therapies for numerous diseases. Preliminary studies performed both in vitro and in vivo suggest that this interaction between MSCs and macrophages plays a substantial role in various biological events, such as tumor growth, immune modulation, tissue restoration, and inflammation regulation. Enhancing our comprehension of this interaction could shed light on possible mechanisms when macrophages and MSCs are used therapeutically. Creative Biolabs combines solid biological knowledge with cutting-edge analysis technologies, delivering a macrophage-mesenchymal stromal cell interaction analysis service for global customers. Based on our robust innovative platforms, our experienced scientists will give high-quality analysis outcomes to progress your projects' execution.

Fig.1 Potential MSC-mediated action pathways on TMφ.¹

Macrophage-Mesenchymal Stromal Cell Interaction Analysis Service at Creative Biolabs

Creative Biolabs provides a macrophage-mesenchymal stromal cell interaction analysis service to assist customers in project development. Macrophages contact with mesenchymal stromal cells directly to regulate each other. On the other hand, they secrete cytokines and other chemical signals to promote communication between them. We provide a set of full-around approaches to characterize their interaction range from morphological to molecular alterations. In addition, we also offer a single-cell sequencing service to elucidate more detailed information about their interactions. With our service, customers not only obtain a throughout insight into their interactions to screen the effective therapeutic targets but also examine the therapeutic efficacy of compounds.

Our cell-based macrophage-mesenchymal stromal cell interaction analysis service can be performed on both primary cells and cell lines:

Primary cells: human-derived, mouse-derived, and other mammals-derived.
Macrophage cell lines: RAW 264.7, J774, BACl.2F5, THP-1, etc.
Mesenchymal stromal cell lines: HS-5, HS-27A, C3H10T1/2, KUSA-A1, etc.

Focused on the phenotypes and molecular alterations, we provide a series of effective methods to characterize:

Tab.1 Analysis methods at Creative Biolabs.

Direct Co-Culture	Characterizing interaction from morphologies.
Flow Cytometry Analysis	Characterizing interaction from cell-surface markers.
Immunocytochemical Analysis	Characterizing interaction from cell-surface markers.
Luminex method	Characterizing interaction from the secretory ability of macrophages and mesenchymal stromal cells.
Transwell Migration Assay	Characterizing interaction based on the migration ability of MSCs from various sources in response to macrophage stimulation.
Live Cell Imaging	Characterizing interaction from the motility of analyzed MSC types influenced by the secretome from different subsets of macrophages.
Single-cell sequencing	Characterizing interaction from transcriptomic or proteomic.

Benefits for You

Competent Ph.D. research team.
Diverse choices to select from.
Rigorous quality control.
One-stop customized service.

The interaction between macrophages and mesenchymal stromal cells is increasingly drawing attention as a therapeutic target. Numerous researches have been made for deep comprehension of their interaction. Please don't hesitate to contact us with your significant interaction analysis projects for more service details.

Reference

Stevens, Hazel Y., et al. "Molecular crosstalk between macrophages and mesenchymal stromal cells." Frontiers in Cell and Developmental Biology 8 (2020): 600160.