The RAW 264.7 macrophage model offers a balance of relevance to macrophage biology, ease of use, reproducibility, and cost-effectiveness, making it a popular choice for studying macrophage-related processes and diseases in vitro. Creative Biolabs specializes in the development of RAW 264.7-based macrophage models for research purposes, and our scientists and technology platform have expertise in macrophage cell culture and model development.

Overview of RAW 264.7 Cells

RAW 264.7 is a murine macrophage-like cell line derived from Abelson murine leukemia virus-induced tumor. This cell line is extensively used in immunology due to its ability to differentiate into functional macrophages, phagocytose particles, secrete cytokines, and respond to inflammatory stimuli.

Fig.1 Macrophage RAW 264.7 cells.^1,3

Key Features of RAW 264.7 Cells

RAW 264.7 cells are valued for their ability to be easily cultured and manipulated in vitro, making them an important tool for investigating various cellular processes and responses. RAW 264.7 cells are favored in immunological and toxicological studies for several compelling reasons.

• Ease of culture: Adherent growth and high proliferation rate
• Strong responsiveness: Robust reaction to lipopolysaccharide (LPS), IFN-γ, and other immune modulators
• M1/M2 polarization capacity: Can be modulated to mimic pro-inflammatory (M1) or anti-inflammatory (M2) states
• Genetic manipulability
• Reproducibility: Consistent phenotype across passages supports reliable data generation

RAW 264.7 models offer a cost-effective and scalable alternative to primary macrophage cultures, making them ideal for high-throughput screening platforms.

RAW 264.7-based Macrophage Model Development Service at Creative Biolabs

Creative Biolabs offers macrophage cell line development services tailored to specific research needs. The following services are based on the RAW 264.7 macrophage model:

Our process includes:

Table 1 Workflow of our service

Creative Biolabs supports flexible customization, including cytokine preconditioning (e.g., IFN-γ, IL-4), time-course stimulation protocols, co-culture systems with fibroblasts, tumor cells, or pathogens, and high-content imaging for real-time cell behavior monitoring.

We ensure each RAW 264.7-based macrophage model meets the highest standards.

• Cell identity authentication
• Mycoplasma testing
• Functional validation
• Viability & growth rate assessments

Each model is delivered with a complete validation report, ready for immediate downstream application. We also offer a wide array of macrophage-related and immune cell modeling services.

• Primary Mouse Macrophage Isolation
• THP-1 Macrophage Model Development
• Human Monocyte-derived Macrophage Models
• Macrophage Polarization Assay Services
• Cytokine Profiling & Secretome Analysis

For a tailored quotation or to discuss your RAW 264.7-based project needs, contact our scientific support team today.

Related Products

After successfully developing a macrophage model, researchers may require high-quality macrophage products to further advance their research. Our company's macrophage product line includes a variety of finely processed macrophages designed to meet the needs of different research directions. These products include, but are not limited to:

Service Features

Phenotypic Versatility

Our RAW 264.7 models can be readily polarized into M1-like macrophages and M2-like macrophages. This enables detailed investigation of both arms of macrophage functionality in health and disease.

Scalability for Screening

RAW 264.7 cells are ideal for HTS， cytotoxicity profiling and dose-response experiments. They grow rapidly, respond uniformly, and can be easily adapted to 96-/384-well formats.

Time & Cost Efficiency

Compared with primary macrophages, RAW 264.7 cells require no animal use or isolation steps, exhibit fast doubling time, and reduce experimental variability and costs.

Comprehensive Data Support

Each delivered model includes cell culture protocols, polarization conditions, marker expression profiles, and functional validation reports.

Publications

RAW 264.7 cells are one of the most recommended cell lines for investigating the activity and differentiation of osteoclasts. RAW 264.7 cells were inoculated in tissue culture polystyrene (TCPS) dishes in proliferation medium and cultured continuously. Cellular mitochondrial activity was determined by MTT assay and cell morphology was observed using scanning electron microscopy (SEM). Experiments were performed to investigate the effects of different concentrations of melatonin on RANKL-induced differentiation of RAW 264.7 cells by adding melatonin directly to osteoblastogenic medium.

Fig. 2 Characterization of RAW 264.7 cells.^2,3

Scientific Resources

Learn More Macrophage Biology

Download Macrophages Development

Learn More Monocyte-Macrophage Development Process

Learn More Macrophage-related Experimental Protocols

Q & A

Q: Can the RAW 264.7 model be used for drug screening? What is the support for throughput?

A: RAW 264.7 is a widely used cell line for drug screening and is suitable for high throughput screening systems. Creative Biolabs supports 96/384 well plate format experiments with automated loading and reading equipment to rapidly assess the effects of drugs on inflammatory pathways, phagocytosis or cytotoxicity. In addition, we can assist in the development of fluorescent or luminescent-based screening systems to improve throughput and data accuracy.

Q: Do you provide complete experimental design and report?

A: Yes, we provide one-stop service, including experimental design, cell processing, data acquisition, statistical analysis, graphical presentation and complete experimental report. The report is detailed and contains experimental methods, raw data, graphical presentation and statistical analysis, which can be directly used for scientific research publication. We also support customized data format and style according to customer needs.

Q: RAW 264.7 is the model applicable to human-related studies? How to explain its species differences?

A: Although RAW 264.7 is derived from mice, it is highly conserved with human macrophages in many pathways, such as NF-κB, MAPK, TLR4, and other inflammatory pathways, and thus is often used as a pre-model for human disease mechanisms. However, it is recommended to combine with human cells or primary models for validation in fine molecular mechanism analysis or in the final stage of drug development. We also provide human macrophage models such as THP-1 and U937 for subsequent validation.

Q: How long is the service cycle typically? How to ensure the cell status?

A: The duration of the experimental cycle depends on the complexity of the project. All of our cells are handled in a sterile environment by an experienced cytology team to ensure cell viability, morphologic consistency and experimental reproducibility. All cell states are examined microscopically and tested for activity before and after the experiment.

References

1. Kang, Sang-Rim, et al. "Suppressive effect on lipopolysaccharide‐induced proinflammatory mediators by Citrus aurantium L. in macrophage RAW 264.7 cells via NF‐κB signal pathway." Evidence‐based Complementary and Alternative Medicine 2011.1 (2011): 248592. https://doi.org/10.1155/2011/248592
2. Jarrar, Hala, Damla Çetİn Altindal, and M. E. N. E. M. Ş. E. Gümüşderelioğlu. "The inhibitory effect of melatonin on osteoclastogenesis of RAW 264.7 cells in low concentrations of RANKL and MCSF." Turkish Journal of Biology 44.6 (2020): 427-436.. https://doi.org/10.3906/biy-2007-85
3. Distributed under Open Access license CC BY 4.0, without modification.