RAW 264.7-based Macrophage Model Development Service

RAW 264.7 macrophage model offers a balance of relevance to macrophage biology, ease of use, reproducibility, and cost-effectiveness, making it a popular choice for studying macrophage-related processes and diseases in vitro. Creative Biolabs specializes in the development of RAW 264.7-based macrophage models for research purposes, and our scientists and technology platform with expertise in macrophage cell culture and model development.

RAW 264.7 Cells

RAW 264.7 is a widely used murine macrophage cell line that originated from tumors induced in BALB/c mice by the Abelson leukemia virus. These cells are commonly utilized in biomedical research, particularly in studies related to immunology, inflammation, and cancer. RAW 264.7 cells are valued for their ability to be easily cultured and manipulated in vitro, making them an important tool for investigating various cellular processes and responses.

Fig.1 Macrophage RAW 264.7 cell visualized by optical microscopy. (Kang, Sang-Rim et al., 2011) Fig.1 Macrophage RAW 264.7 cells.1

RAW 264.7-based Macrophage Model Development Service at Creative Biolabs

Creative Biolabs offers macrophage cell line development services tailored to specific research needs. The following services are based on the RAW 264.7 macrophage model:

The above is the basic framework for macrophage model development services based on RAW 264.7. In addition to that, we have many more services for macrophage therapy, you can contact us directly for more information!

Display Results

RAW 264.7 cell morphology
Fig.2 Morphology of RAW 264.7 cells under electron microscopy. (Teng, Liping et al., 2015)
Fig.2 RAW 264.7 cell morphology.2
Morphology of RAW 264.7 cells after treatment using different methods.

Frequently Asked Questions

Q1: Why choose the RAW 264.7 macrophage model?

A1: Below we list some of the advantages of the RAW 264.7 macrophage model:

  • RAW 264.7 cells share many characteristics with primary macrophages, including phagocytic activity, antigen presentation, cytokine production, and response to inflammatory stimuli.
  • RAW 264.7 cells are immortalized, providing a consistent and easily accessible cell source for experiments, reducing the need for repeated isolation of primary cells.
  • RAW 264.7 cells are relatively easy to culture and maintain compared to primary macrophages.
  • RAW 264.7 cells exhibit relatively consistent behavior, reducing variability in results compared to primary cells, which can vary due to donor differences and isolation procedures.
  • Using a cell line like RAW 264.7 eliminates the need for animal procurement, housing, and specialized reagents for primary cell isolation.

Q2: What should I do if RAW 264.7 cells grow slowly?

A2: Regular passaging and control the proportion of passaging. Perform mycoplasma testing promptly, and if contamination is found, remove it immediately with mycoplasma removal reagents.

If your project requires macrophage models, you can work with Creative Biolabs with confidence. We will be your most reliable partner. For more information on macrophage models, you can click here.

References

  1. Kang, Sang-Rim et al. "Suppressive effect on lipopolysaccharide-induced proinflammatory mediators by citrus aurantium L. in macrophage RAW 264.7 cells via NF-κB signal pathway." Evidence-based complementary and alternative medicine. 2011 (2011): 248592.
  2. Teng, Liping et al. "Stimulation of RAW264.7 macrophages by sulfated Escherichia coli K5 capsular polysaccharide in vitro." Molecular medicine reports. 12,4 (2015): 5545-53.
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