In vivo lentivirus-based macrophage reprogramming typically involves genetic modification of macrophages using lentiviral vectors. This approach provides a powerful tool to explore and utilize the potential of macrophages in various biological and medical applications. As a pioneer in the tumor-immunity field, Creative Biolabs integrates lentivirus-based cell modification with macrophage reprogramming and successfully developed a series of in vivo lentivirus-based macrophage reprogramming services to assist the application of macrophage for disease treatment.

Lentivirus-based Modification: Inclusive! Steady! Safe!

Lentiviral vectors are the preferred choice for in vivo reprogramming techniques because they offer the following advantages.

• Highly efficient gene transfer capability, allowing stable integration of target genes into the cellular genome
• Low immunogenicity, which reduces adverse reactions and immune rejection
• High adaptability to a wide range of immune cells, including macrophages

Macrophage reprogramming utilizes lentiviral vectors that can efficiently introduce specific genes or regulatory molecules to reprogram macrophages. This process can be carried out in an in vivo environment, ensuring that the experimental results are closer to the physiological state and greatly enhancing the relevance and validity of the research.

In vivo lentivirus-based macrophage reprogramming services are widely used in the following areas:

• Immunology research: to help researchers reveal the role of macrophages in immune response.
• Disease modeling: to explore the functional changes of macrophages in pathological states such as tumors, infections and autoimmune diseases.
• Drug development: to assess the effects of new drugs on macrophage function and provide an experimental basis for the development of new therapies.

In Vivo Lentivirus-based Macrophage Reprogramming Services at Creative Biolabs

We utilize the lentivirus vectors to deliver the desired gene into the macrophage for in vivo engineering purposes. Throughout the process, we provide comprehensive customized services from lentivirus production to results visualization to meet global customers' diverse needs.

In term of different diseases and targets, we provide several related services and diverse target genes that can be chosen from for your projects:

Importantly, we also provide a lentivirus production service featuring strict quality control to deliver high-quality lentivirus productions for global customers.

Key features of this service:

• Efficient introduction: Lentiviral vectors have excellent transfection efficiency and are able to stably express target genes in various cell types, ensuring that macrophages can effectively respond to reprogramming signals.
• Customized service: The reprogramming service we provide supports the introduction of multiple genes, which can be customized according to customer's research needs, helping researchers to deeply explore the role of different genes in macrophage function.
• Experimental support: We not only provide virus production and purification services, but also can provide customers with a full range of technical support such as experimental protocol design, data analysis, etc. to ensure the smooth progress of experiments.
• Strict quality control: All viral vectors are produced in laboratories that comply with relevant biosafety standards to ensure the safety and effectiveness of the products and avoid any potential impact on the experimental results.

As a pioneer in the field, Creative Biolabs is committed to innovating technologies to aid in the development of disease treatment and improve human life quality. Here we provide an inquiry mode for your convenience to contact us. We are glad to receive your inquiry to know more about in vivo lentivirus-based macrophage reprogramming services.

Related Products

High-quality lentiviral vectors are required for efficient reprogramming of macrophages in vivo. Our products provide a solution to this need. By combining advanced macrophage reprogramming techniques with efficient tools, researchers can explore the role of macrophages in disease more comprehensively, laying the foundation for the development of new immunotherapeutic strategies.

Service Features

Publications

Chimeric antigen receptor macrophage (CAR-M) therapy is a novel cancer immunotherapy that integrates CAR structure and macrophage function. Yi Huo et al. constructed a novel HER2-targeted CAR-M that was able to efficiently eliminate HER2-positive tumor cells both in vivo and in vitro, and M1 polarization significantly enhanced the anti-tumor ability of CAR-M.

In their experiments, they synthesized anti-HER2 CAR sequences and cloned them into lentiviral vector backbones, followed by virus production in cells using a third-generation lentiviral packaging system. The lentiviral supernatant was concentrated and added to mouse BMDM for transduction.

Fig. 1 Overview of primary macrophage differentiation from bone marrow and the lentivirus transduction protocol.^1,2

Scientific Resources

Learn More Macrophage Biology

Download M1 vs. M2 Macrophages

Learn More Tumor-Associated Macrophages

Learn More Isolation and Identification of Macrophages

Q & A

Q: What research areas are your services applicable to?

A: Our macrophage reprogramming service is applicable to a number of research areas, including basic immunology, cancer research, inflammatory diseases, infection modeling, and regenerative medicine. Whether you wish to explore the role of macrophages in a specific disease or develop new therapeutic strategies, all are scenarios where our service is applicable.

Q: How long do reprogrammed macrophages last?

A: The survival time of reprogrammed macrophages varies depending on the experimental conditions, and typically these cells can continue to proliferate and function for weeks to months under appropriate culture conditions. We recommend assessing the cell status periodically to ensure that the experiment runs smoothly.

Q: What support can you provide to assist customers in designing experiments?

A: We provide comprehensive experimental design support, including consulting services to identify optimal reprogramming genes, select appropriate cell types, optimize infection conditions, and develop subsequent experimental procedures. Our scientific team will provide personalized advice and guidance based on the client's research objectives to ensure that the experimental design is sound and can achieve the desired results.

Q: Can customers select specific genes for reprogramming?

A: Yes, customers can customize the genes they want to reprogram according to their research needs. We will assist customers in evaluating the suitability of the selected genes and provide relevant technical advice to ensure that the selected genes can effectively achieve the desired reprogramming effect.