The Importance of CNS Macrophages for Neuro-disease

Macrophages serve an essential part in both the formation and development of neuro-diseases. In the central nervous system (CNS), microglia and three kinds of border-associated macrophages (BAMs) make up the CNS macrophage population. These cells play a crucial role in maintaining homeostasis and have a direct impact on both the growth and recovery of neuroinflammatory processes. Research evidence showed that these cells are significant contributors to CNS disorders, including autoimmune diseases like multiple sclerosis, as well as degenerative diseases like Alzheimer's disease. Therefore, macrophages hold great potential as a viable target for treating neurological diseases.

Fig.1 Brain macrophage categorization in disease and health.¹

Our In Vivo Lentivirus-based Macrophage Reprogramming Service for Neuro-disease

At present, considerable progress has been achieved in the gene-mediated therapy of neurological diseases. This progress can be attributed to i) a better understanding of the mechanisms underlying these diseases, ii) improved methods for introducing exogenous gene vectors, and iii) advancements in gene transfer technology.

Based on these, Creative Biolabs developed a safe and effective in vivo lentivirus-based macrophage reprogramming service for neuro-disease to boost the application of macrophages to neurological disease treatment. We utilize a lentiviral vector with low cytotoxicity and immunogenicity to load the diverse therapeutic target genes, and deliver the recombinant vector to the target macrophages in vivo. Throughout the process, we provide comprehensive services in accordance with the diverse demands of global customers to enable the desirable results for each of you.

Fig.2 The lentiviral vector's genomic and structural layout.²

Hot Targets

Here are some hot targets that have been well-studied.

Key Advantages: Guarantee for High-quality Outcomes

• Significant capacity of transferred genes.
• Long-term expression of target genes.
• Lower cytotoxicity and immunogenicity.
• Years of project experience.
• Strict control of quality.

Case Show

Case 1:

Targets: BNDF

Background: Brain-derived neurotrophic factor (BDNF) has emerged as a promising candidate for enhancing neuroprotection, axonal regeneration, and synaptic plasticity in the aftermath of spinal cord injury (SCI). The influence of BDNF on the inflammatory response during the progression of secondary injury after SCI is still unknown.

Method: This study investigated the effect of BDNF on macrophage polarization after spinal cord injury by loading BNDF onto a lentiviral vector and then slowly injecting the recombinant vector into the injured site.

Results: The findings suggest that BDNF may have the ability to improve the inflammatory microenvironment and potentially induce a shift from the M1 to M2 phenotype. The immunomodulatory functions of BDNF include not only the potential neuroprotective benefits, but also contributing to the functional recovery of locomotion following spinal cord injury.

To enhance cancer treatment and quality of life in general, Creative Biolabs, an industry pioneer, relies on years of invaluable experience and an extensive range of professional knowledge to develop innovative technologies.

We provide an in vivo lentivirus-based macrophage reprogramming service for neuro-disease, and if you are interested in learning more about it, please get in touch with us.

References

1. Silvin, Aymeric, et al. "Brain macrophage development, diversity and dysregulation in health and disease." Cellular & Molecular Immunology (2023): 1-13.
2. Dong, Wendy, and Boris Kantor. "Lentiviral vectors for delivery of gene-editing systems based on CRISPR/Cas: current state and perspectives." Viruses 13.7 (2021): 1288.