Monocyte Products

Monocytes are bone marrow-derived leukocytes that circulate in the blood and spleen. Characterized by their ability to recognize “danger signals”, monocytes can phagocytose and present antigens, secrete chemokines, and proliferate in response to infection and injury. Once recruited to tissues, monocytes are capable of differentiating into macrophages and dendritic cells. Monocytes and macrophages are key components of the innate immune system and are involved in the regulation of the initiation, development, and resolution of many inflammatory disorders.

Moreover, these cells also play important immunoregulatory and tissue-repairing roles to decrease immune reactions and promote tissue regeneration. Furthermore, they also contribute to a broad spectrum of pathologies and are thus attractive therapeutic targets.

Creative Biolabs has developed a range of high-quality monocytes by positive or negative selection from different species and sources. When commercially available off-the-shelf (COTS) products are not enough, trust Creative Biolabs to develop a solution that will provide the best results you require. Please don't hesitate to contact us and see how we can help you with your project.

Related Services

Scientists at Creative Biolabs are versed in the development of macrophage therapeutics. We are glad to serve our global clients with professionalism and expertise. Our comprehensive portfolio provides reliable support to help our clients' macrophage therapeutics development projects. In addition to a range of useful products, we also provide a full range of high-quality services.

Creative Biolabs offers a comprehensive portfolio of services including macrophage isolation and culture services, macrophage polarization assays, macrophage polarization phenotyping and characterization services, macrophage reprogramming services, macrophage model development services and more. You can browse below and click to learn more.

Publications

Elza Evren et al. developed a humanized mouse model that supports reconstitution of human blood monocyte and lung macrophage compartments after transplantation of human CD34⁺ hematopoietic stem and progenitor cells (HSPC). They used a humanized mouse model combined with progenitor cell transplantation, fate localization, intravascular cell labeling, and single-cell RNA sequencing to define the individual development of human macrophages from precursors to mature lung macrophages.

Results showed that classical CD14⁺ CD16^- monocytes were identified as circulating precursors of lung tissue monocytes as well as interstitial and alveolar macrophages. In contrast, nonclassical CD14^lo CD16⁺ monocytes were restricted to the pulmonary vascular system and gave rise to a distinct population of pulmonary intravascular macrophages.

Fig. 1 Distinct developmental pathways from blood monocytes generate human lung macrophage diversity.¹

Applications

We offer monocyte products for research use, which can substantially aid researchers in various fields including immunology, cell biology, and drug development.

• Immunological Research
  
  • Immune Response Studies: Researchers can use our products to study the activation, differentiation, and function of monocytes in response to various stimuli, such as pathogens and cytokines.
  • Cytokine Production: Our products enable researchers to measure cytokine production and understand its role in inflammation and immune regulation.
• Macrophage Polarization
  Researchers can utilize monocyte products to investigate the polarization of monocytes into different macrophage phenotypes under various conditions. This is essential for understanding their role in diseases, including cancer, and in tissue repair mechanisms.
• Disease Modeling
  
  • Inflammatory Diseases: Researchers can study the contribution of monocytes to inflammatory disease pathology and test potential therapeutic interventions.
  • Cancer Research: Our products support studies that assess monocyte-tumor interactions and their impact on cancer progression.
• Drug Development and Testing
  
  • Toxicology Studies: Monocytes can be used to evaluate the immunological impact of new drugs, helping researchers assess toxicity profiles and safety in preclinical trials.
  • Therapeutic Targets: Identifying monocyte-related pathways in disease states can lead to the development of targeted therapies. Our products facilitate the study of these pathways and potential drug candidates.

Scientific Resources

Q & A

Q: How should I handle monocytes once I receive them?
A: Upon receiving monocytes, it is crucial to handle them with care to maintain their viability and functionality. First, check the integrity of the packaging and the temperature of the samples. If they arrive on ice, they should be stored in a refrigerator (2-8℃) until use. Always use sterile techniques while handling to prevent contamination, and follow specified protocols for cell culture and experimentation to ensure accurate results.

Q: What types of experiments can I perform using these monocytes?
A: Monocytes can be used in a variety of experiments, including but not limited to in vitro studies on cell signaling, cytokine production, and phagocytosis. They are also suitable for assays assessing immune responses, differentiation into macrophages, and interaction with pathogens. Furthermore, researchers can utilize them in drug testing to evaluate the immunological effects of new compounds and study their role in disease pathology.

Q: How long can I keep the monocytes before use?
A: Monocytes are best used soon after receipt to ensure optimal functionality. If stored under proper conditions, they can generally be kept for 3-5 days in refrigeration. However, prolonged storage beyond this timeframe may lead to decreased viability and altered functional characteristics. For longer-term studies, consider cryopreservation techniques immediately upon receipt to preserve their quality for future experiments.

Q: Can you customize the monocytes for specific research needs?
A: We offer customized services for monocytes, including modifications to culture conditions or selection based on specific surface markers. If you have specific requirements, it's advisable to communicate your needs directly to us who can guide you on available options.

Q: What should I do if the monocytes arrive damaged or of poor quality?
A: If you receive monocytes that appear damaged or of poor quality, it is important to contact us immediately. Provide us with details about the condition of the shipment, including any photos if possible. We have a customer service policy that includes replacement or credit for compromised products. Documenting your findings will facilitate a smoother resolution process.

Customer Reviews

Reference

1. Evren, Elza, et al. "Distinct developmental pathways from blood monocytes generate human lung macrophage diversity." Immunity 54.2 (2021): 259-275, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/).