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Human PB CD14+ Monocytes (Age: 28), 2.5 x 10^7 cells (MTS-1022-JF19)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Is this format pre-enriched, and what are my options if I need higher CD14+ purity or a less manipulated population?

This page represents a general PB CD14+ monocyte offering without an explicit selection label. If your assay benefits from higher enrichment, we can discuss positive selection formats; if you want minimal surface perturbation, negative selection may be more appropriate. Tell us your readouts and we'll recommend the best fit to reduce confounding and improve reproducibility.

Can this quantity (2.5 × 10^7 cells) support parallel workflows (e.g., differentiation + functional panel + RNA), and how should I allocate cells?

To avoid donor mixing, plan allocations before thaw: reserve a fraction for quick phenotype confirmation, dedicate a defined portion to your primary workflow, and keep a small contingency for repeats. For multi-plate designs, staging the workflow and minimizing time in concentrated suspension improves consistency across plates and timepoints. With 2.5 × 10^7 cells, most teams can run parallel arms if allocation is defined up front.

If we need to repeat the experiment in a later phase, how do you help us keep results comparable across lots?

If you anticipate reorders, we can support continuity planning by recommending ordering cadence, lot strategy, and anchoring controls that keep datasets comparable.

The major advantage was operational-one vial supported the entire experimental matrix without mixing donors
Post-thaw recovery was strong, and clumping was manageable with gentle handling and a single additional wash step. Our differentiation into macrophage-like cells was predictable and the baseline was not "over-activated," which mattered for downstream stimulation.
This high-count format made it easy to scale a multi-plate screening workflow
We ran uptake and phagocytosis-style readouts in parallel with flow panels and kept enough cells for repeat runs, all from one donor lot. The cells tolerated recovery well, and we saw consistent responses across plates when we standardized seeding density and resting time.
Our group uses monocytes as the starting material for macrophage differentiation
With this batch, the initial phenotype checks were clean and the cells were robust through early culture. We appreciated that the supplier understood how subtle processing differences can shift baseline cytokine levels. The guidance on resting and media changes resulted in a stable baseline, which improved the interpretability of our stimulation panel.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.