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Human PB CD14+ Monocytes (Age: 31), 5 x 10^7 cells (MTS-1022-JF24)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What should I do immediately after delivery to protect viability?

Treat this as a cold-chain-critical shipment: move the vial directly from dry ice into liquid nitrogen storage (or keep on dry ice only briefly while preparing LN2 access). Viability loss is most commonly caused by temperature cycling during receipt. When you're ready to use it, thaw quickly, dilute dropwise into warm medium, and minimize centrifugation stress.

Are these cells suitable for downstream macrophage polarization (M1/M2) and cytokine profiling?

Yes. CD14+ monocytes are a standard precursor for macrophage differentiation and polarization workflows. Success hinges on tight control of baseline activation: use low-endotoxin reagents, equilibrated media, and consistent serum/lipid components. If your readout is cytokine-heavy, include unstimulated controls and an early "baseline" sampling point after plating.

Can I use them for monocyte-to-DC differentiation as well?

Absolutely. Many teams split the same monocyte starting material into macrophage and DC arms to compare antigen presentation, migration, and cytokine signatures. Plan your experiment so the thawed cells recover overnight (when possible), then begin DC differentiation with defined cytokines on a consistent schedule to reduce maturation variability.

For time-course experiments, the high-count format translated into cleaner comparisons and less hidden variability
We allocated cells for all timepoints from the same donor input, which improved our confidence in the kinetics. Recovery was strong and the cells handled well when we split the wash steps into multiple tubes. Baseline behavior stabilized after a consistent rest, and stimulation responses were reproducible across plates. The supplier's workflow advice (staging, minimizing bench time) was especially relevant at this scale.
We used this batch for a large plate-based screening workflow
The advantage of the 5 × 10^7 format is that you can run a big matrix without mixing donors, which reduces noise. The cells were robust post-thaw and remained responsive across plates when we standardized seeding density and rest timing. The supplier's suggestions on preventing clumping in large suspensions were practical and saved us troubleshooting time.
In large designs, operational details can quietly undermine data quality-this product and the associated support helped us maintain consistency and produce a cleaner dataset
The cells arrived in excellent condition and recovered with strong viability. We ran multiple conditions with adequate replicates and still had enough cells for validation assays. Importantly, the baseline remained controlled, which improved the interpretability of our stimulation panel. The supplier also provided realistic recommendations for staging large plating sessions and keeping timing consistent.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.