The Importance of Mass Cytometry-Based Phagocytosis Assay in Macrophage Characterization

Creative Biolabs offers a macrophage characterization service by mass cytometry-based phagocytosis assay which allows for the investigation of phagocytic activity in different macrophage subpopulations. This service provides a comprehensive analysis of macrophage plasticity by linking cell phenotype to phagocytic behavior, enabling a more informative characterization of macrophage function. Moreover, this assay enables the assessment of signaling reactions in macrophages after engulfing diverse targets. Combined with our macrophage therapeutics development platform, our innovative macrophage characterization service has more potential to serve as a great useful tool to enable a detailed understanding of macrophages in vitro.

Fig.1 Variety in macrophages' characteristics.¹

Macrophage Characterization Service by Mass Cytometry-Based Phagocytosis Assay at Creative Biolabs

Mass cytometry assay combines mass spectrometry technology with flow cytometry to enable an in-depth study of the biochemical components of single cells. Based on it, the macrophage characterization service provided by Creative Biolabs incorporates a cutting-edge mass cytometry-based phagocytosis assay, which allows for the concurrent analysis of phagocytic events along with the expression of up to 40 different protein markers. Importantly, we also supply a macrophage marker development service to aid in rapidly identifying macrophages.

In addition, to help customers' macrophage projects perform smoothly, we also deliver a well-developed service to isolate macrophage cells from mammals and culture them with strict quality control. Together with our seasoned research teams and robust platform, we are confident in presenting each customer with relatively complete macrophage identification results.

Fig.2 Workflow of mass cytometry-based phagocytosis assay.²

Here we list several mass cytometry markers to conveniently study macrophages:

Tab.1 Mass cytometry markers.

Advantages

• Single-cell level molecular profiling: Our macrophage characterization service by mass cytometry-based phagocytosis assay allows investigation into the biochemical constituents of specific cells, enhancing our understanding of their unique biological characteristics.
• Quantitative analysis of biomolecules: Our macrophage characterization service by mass cytometry-based phagocytosis assay provides accurate measurement and assessment of biomolecules present in cells, covering a wide range of molecules such as proteins, metabolites, and various other biomolecules.
• Characterization of cellular heterogeneity: Our macrophage characterization service by mass cytometry-based phagocytosis assay offers advanced techniques for characterizing the heterogeneity of cell populations, allowing for a comprehensive understanding of cellular variations within different cell types.

As an industry-leading company in macrophage characterization, Creative Biolabs is constantly improving our services to deliver more comprehensive information about macrophages. For more details about our macrophage characterization service by mass cytometry-based phagocytosis assay, please don't hesitate to get in touch with us. Welcome your inquiry and order.

References

1. Lavy, Margot, et al. "Specialized pro-resolving mediators mitigate cancer-related inflammation: role of tumor-associated macrophages and therapeutic opportunities." Frontiers in Immunology 12 (2021): 702785.
2. Schulz, Daniel, et al. "In-depth characterization of monocyte-derived macrophages using a mass cytometry-based phagocytosis assay." Scientific Reports 9.1 (2019): 1925.