Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, Colorimetric (MTS-1123-HM49)

This kit supports multiple matrices, including cell culture supernatant, cell lysate, plasma, serum, and tissue homogenate, making it suitable for integrated studies that compare secreted and tissue-associated MIF. To run mixed matrices in one project, we recommend validating each matrix with a dilution linearity check, then using matrix-matched controls so comparisons are meaningful. If your tissue homogenates are complex, clarify them thoroughly and pilot different dilutions to reduce background while keeping recovery acceptable.

The kit is designed for an approximately 3-hour assay and uses a pre-coated 96-test plate with a 100 μL sample volume per well. To avoid common mistakes, keep timing consistent across standards and samples, use fresh wash buffer, and avoid letting wells dry during steps. Plan your plate map in advance and include duplicates for standards and critical samples. Consistent washing and careful pipetting are the biggest drivers of reliable signal-to-noise.

The kit lists storage at 4°C and -20°C (depending on component; follow the protocol) and an expiry of 6 months. For partial usage, aliquot reagents that are prone to repeated freeze-thaw, label opening dates, and store components exactly as instructed in the protocol. We also recommend planning batch runs so standards and controls remain consistent across plates. If you share your intended schedule, we'll suggest a storage and aliquoting plan that reduces waste and maintains performance.