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Macrophage Colony Stimulating Factor (MCSF) ELISA Kit, Fluorometric (MTS-1123-HM7)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Colony Stimulating Factor 1 Receptor (MCSF) in different sample types by Fluorometric.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human, Mouse, Rat

Detection Method

Fluorometric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Cells

Specificity

Colony Stimulating Factor 1 Receptor (MCSF)

Binding Specificity

Serine/Threonine Protein Kinase (STK)

Specification

Size

96 tests

Detection Range

At least 5000 cells/well

Assay Time

4.5 h

Plate

Uncoated

Bioassay Target Name

Colony Stimulating Factor 1 Receptor (MCSF)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

colony stimulating factor 1

Synonyms

MCSF; CSF-1; PG-M-CSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.

FAQs Customer Reviews Related Products

I'm deciding between colorimetric and fluorometric versions. What practical advantages does fluorometric detection give?

Fluorometric detection can offer improved sensitivity and dynamic range in some workflows, and it may better tolerate mildly colored samples that interfere with absorbance readings. If your samples are low-abundance or have intrinsic color/turbidity, fluorometric readout can be beneficial. The main tradeoff is that you'll need a plate reader with appropriate fluorescence capability and consistent settings across runs to maintain comparability.

How do I avoid fluorescence drift or instrument-to-instrument variability if multiple readers are used?

To minimize variability, use the same reader whenever possible and lock down gain, integration time, and any calibration parameters. Include a consistent internal control sample on every plate, and run a small set of standards to confirm the curve looks similar across days. If multiple instruments are unavoidable, cross-calibrate by reading the same plate on both instruments and applying a correction factor validated by control samples.

Are there common buffer components that quench fluorescence and reduce signal?

Some components (high salt, certain detergents, and strongly colored additives) can alter fluorescence background or quench signal. The most practical approach is to test your buffer in a blank well and in a spike recovery format using representative samples. If quenching is suspected, dilute samples into a compatible diluent, reduce interfering additives, and keep handling consistent across groups. Proper plate protection from light during incubations can also improve stability.

Fluorometric readout helped with low-abundance MCSF samples
We chose the fluorometric kit because our macrophage supernatants had relatively low signal in pilot colorimetric assays. With the fluorometric format, we obtained a clearer separation between conditions and less interference from slightly colored media. Instrument settings mattered, so we standardized gain and kept all plates read on the same device. Once set up, reproducibility was strong and the assay scaled well for our screening workflow.
Great option when absorbance is affected by sample coloration or turbidity
Some of our samples had mild turbidity that complicated absorbance readings. The fluorometric kit reduced that issue and made the data easier to interpret. We included a pooled reference control across plates and results were consistent across runs. The protocol felt similar to standard sandwich ELISA handling, just with fluorescence reading requirements. If your plate reader supports it, this version is very convenient.
Reliable performance after we optimized buffer compatibility and light protection
At first we saw higher background, which improved after diluting samples into a cleaner buffer and protecting plates from ambient light during key incubation steps. After that adjustment, replicate variability decreased and the curve was stable. The kit helped quantify relative differences in MCSF across treatments. I recommend doing a short pilot to confirm your matrix doesn't quench fluorescence, then it's smooth sailing.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.