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Macrophage Chemokine Ligand 1 (CCL1) ELISA Kit, qPCR (MTS-1123-HM90)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 1 (CCL1) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 1 (CCL1)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 1 (CCL1)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 1

Synonyms

P500; SISe; TCA3; I-309; SCYA1

Background

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM900	Human	0.4 pg/mL		Inquiry
MTS-1123-HM901	Mouse	3 pg/mL		Inquiry

FAQs Customer Reviews Related Products

Does this kit help me correlate secreted CCL1 protein levels with transcriptional changes, or will I still need to design my own qPCR workflow?

This product format is designed to support both protein- and mRNA-level readouts in one project plan, so you can trend CCL1 secretion alongside gene expression changes after stimulation or inhibitor treatment. In practice, you can run ELISA on supernatants while running qPCR on matched cell pellets, enabling stronger biological interpretation and easier figure building. We also provide technical support for sample handling and normalization strategy suggestions.

What sample types are typically compatible, and how do I avoid matrix effects when measuring CCL1 in complex samples?

The kit is intended for common research matrices such as cell culture supernatant and other biological fluids or lysates where CCL1 is expected to be present. To minimize matrix effects, we recommend using proper dilution series, including spiked recovery checks, and ensuring consistent sample buffers across your standard curve and unknowns. If you share your sample background and expected concentration range, we can suggest practical dilution starting points and controls.

I'm worried about run-to-run variability. How does the kit help ensure reproducibility across multiple plates and time points?

For time-course or multi-plate studies, reproducibility is improved by using consistent pipetting practice, strict incubation timing, and running internal controls across plates (for example, a pooled reference sample). We recommend building an inter-plate normalization plan before you start, especially when comparing different donors or stimulation conditions. If needed, we can help you plan plate maps, replicate strategy, and acceptance criteria so your longitudinal CCL1 trends remain defensible.

Reliable CCL1 quantification and smoother data integration with qPCR
We used this kit to track macrophage activation across multiple stimulations and appreciated the ability to connect secreted CCL1 trends with gene expression changes from matched samples. The workflow was straightforward and the signal separation between treated and control conditions was clear. Customer support was responsive when we asked about dilution strategy for a higher background matrix, and their suggestions helped us tighten variability between replicates.
Good sensitivity for culture supernatants and consistent standard curves
In our hands, the standard curve behaved consistently across runs, which made it easier to compare multiple experiments performed on different days. The kit fit into our existing ELISA schedule without needing special equipment beyond a standard plate reader. We also liked that the documentation helped our junior staff avoid common pitfalls like inconsistent wash steps. Overall, it reduced troubleshooting time and improved confidence in the CCL1 readout.
Helpful technical guidance and practical workflow for activation studies
We were running a macrophage polarization study and wanted a dependable CCL1 readout to complement flow markers. The kit provided clean results once we optimized sample dilution, and the vendor's guidance on using pooled controls across plates was genuinely useful. Turnaround was good and the reagents were packaged clearly. I'd recommend it for groups that want publishable CCL1 trends without excessive optimization.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.