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Macrophage Elastase (MMP12) ELISA Kit, qPCR (MTS-1123-HM48)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Macrophage Elastase (MMP12) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sensitivity

0.004 ng/mL

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Macrophage Elastase (MMP12)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Macrophage Elastase (MMP12)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

matrix metallopeptidase 12

Synonyms

ME; HME; MME; MMP-12

Background

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11.

FAQs Customer Reviews Related Products

We need to screen many conditions quickly. Is this qPCR-readout ELISA better for screening than colorimetric ELISA, and why is it described as semi-quantitative?

This kit is described as a sandwich ELISA with qPCR readout for semi-quantitative measurement of MMP12. It can be a good screening tool when your goal is ranking conditions (higher vs lower expression) rather than reporting strict absolute concentrations. Semi-quantitative positioning reflects how results are typically interpreted-relative differences with defined controls. For large screens, we recommend consistent plate controls and a reference sample to normalize across runs so week-to-week comparisons remain meaningful.

If I want to correlate protein results with MMP12 enzymatic activity, what do you recommend to ensure the conclusions are sound?

Protein abundance and enzymatic activity are related but not identical, especially for proteases with pro/active forms and inhibitor interactions. We recommend using this kit to quantify relative protein-level changes, then pairing it with an activity-focused method (activity assay or zymography) on a subset of key samples. This staged approach is efficient: screen broadly for changes, then validate mechanistic activity where it matters. We can help plan a two-tier workflow, including which samples to prioritize and what controls to include so the correlation is interpretable.

How do you help customers establish acceptance criteria so screening data are reproducible and comparable across weeks?

We suggest defining acceptance criteria before you start: replicate CV limits, standard/control performance thresholds, and rules for reruns when curves deviate. Because the assay is semi-quantitative, consistent internal controls become especially important-include a pooled reference sample and track its performance over time. If you share your throughput and expected variability, we can propose a practical QC checklist and plate layout strategy to keep long screening campaigns consistent.

Strong screening utility for relative MMP12 changes across conditions for consistent, publication-ready reporting
We used this kit as part of a larger screening campaign and treated the output as semi-quantitative, focusing on ranking conditions rather than absolute concentration claims. With consistent controls and a pooled reference sample, the data were stable across multiple weeks. The qPCR readout fit our lab infrastructure and helped avoid some issues we had with borderline colorimetric signals. It's a useful tool when your goal is screening and prioritization for follow-up validation.
Helpful for selecting samples to run expensive activity assays afterward
We needed a method to decide which conditions warranted deeper protease activity testing. This kit gave clear relative differences and helped us narrow down candidates efficiently. The vendor advised on setting acceptance criteria and using internal controls to minimize cross-run drift, which improved confidence. Once we selected top conditions, we ran an activity assay and saw alignment in most cases. Overall, it supported a practical two-step workflow.
Consistent results once we standardized plate handling and timing in our inflammation assay pipeline
The first run was good, but we improved consistency further by standardizing incubation timing and having one operator handle key steps. After that, replicate variability decreased and condition-to-condition differences were reproducible. We appreciated that the kit description and support team emphasized semi-quantitative interpretation, which helped us present the screening results appropriately. It's a solid option if you need dependable relative MMP12 readouts at scale.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.