The Importance of Cytokine Expression in Macrophage Therapeutics

Cytokines serve a crucial role in macrophages, functioning as pioneers of the body's innate immune system and facilitating the shift from innate to adaptive immunity. Examining the differences in cytokine expression profiles across various macrophages could provide valuable insights into their unique characteristics, which could prove crucial for studies pertaining to macrophages. Together with our experienced research teams and innovative platforms, the cytokine expression profiling service provided by Creative Biolabs has the potency to help customers profile the cytokine expression of target macrophages treated with interest compounds.

Fig.1 Monocytes may differentiate into several types of macrophages.¹

Cytokine Portfolio for Macrophages

• Proinflammatory cytokines: tumor necrosis factor (TNF), IL-1, IL-6, IL-8, IL-12, IL-18, IL-23, and IL-27.
• Anti-inflammatory cytokines: IL-10, TGF-β, CXCL1 and CXCL2 (MIP-2α), CCL5 (RANTES), CXCL8 (IL-8), CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (IP-9).

Cytokine Expression Profiling Service by Real-Time PCR Analysis or Microarray Analysis at Creative Biolabs

The cytokine expression profiling service from Creative Biolabs takes advantage of the real-time PCR analysis or microarray analysis approaches, which have the capacity to perform sensitive and high-throughput profiling of macrophages' cytokine expression. Our RT-PCR may spot cytokine mRNA levels in particular cells, and our microarray analysis allows for determining the expression levels of several genes at the same time. Based on them, we not only deliver detection services about certain cytokines or specific groups of cytokines but also customize solution services for customers who have any other specific demands. At Creative Biolabs, we will try our best to advance your macrophage projects to progress smoothly.

Importantly, our cytokine expression profiling service by real-time PCR analysis or microarray analysis is suitable for:

• Primary macrophage
• Macrophage cell lines

Here we provide multiple types of stimuli to design for your macrophage projects:

Fig.2 Diverse types of stimuli for macrophage development.

Features

• Cost-effective macrophage cytokine expression solutions.
• Rapid screening of crucial information on cytokine expression of macrophages.
• Highly reliable and reproducible data delivery.
• All-inclusive services.

Data Speaking

DATA: This study aimed to investigate the changes in cytokine expression in bone marrow, spleen, and peritoneal macrophages using real-time PCR under both resting and lipopolysaccharide (LPS)-stimulated conditions. The findings demonstrated that the expression levels of cytokines differed across the three types of macrophages under both states. Resting bone marrow macrophages (BMs) exhibited significantly higher expression of TGF-β and IL-10 compared to spleen macrophages (SPMs) and peritoneal macrophages (PMs). This suggested that BMs generated in vitro might have a greater likelihood of displaying an M2 phenotype. On the other hand, pro-inflammatory cytokines such as IL-6, IL-12, and TNF-α were significantly more highly expressed in both activated and non-activated SPMs compared to BMs and PMs. These results highlighted the distinct biological functions of the three types of macrophages based on their cytokine expression patterns.

Fig.3 The cytokine expression of three types of macrophages under resting and LPS-stimulated conditions.²

As a leading company in macrophage characterization, Creative Biolabs strives to develop useful technologies to aid in customers' diverse projects. If you are interested in our cytokine expression profiling service by real-time PCR analysis or microarray analysis, please feel free to contact us.

References

1. Arango Duque, Guillermo, and Albert Descoteaux. "Macrophage cytokines: involvement in immunity and infectious diseases." Frontiers in Immunology 5 (2014): 491.
2. Wang, Changqi, et al. "Characterization of murine macrophages from bone marrow, spleen and peritoneum." BMC Immunology 14.1 (2013): 1-10.