Macrophage Isolation and Culture Service
Our Service Related Products Service Features Workflow Publications Scientific Resources Q & A
Macrophages are able to initiate a particular type of inflammatory response via the release of a certain pattern of cytokines. They play a major role as immunoregulatory cells. Creative Biolabs has built a highly experienced team of scientists and quality staff that have a long history in macrophage isolation and culture. Human monocyte, human alveolar, murine peritoneal cavity, murine bone marrow, murine lung, and murine adipose tissues are available for macrophage isolation and culture. Our seasoned scientists are pleased to share our expertise and experience with our global clients and facilitate their meaningful macrophage project development.
Fig.1 Workflow of monocyte isolation, and macrophage differentiation and polarization.1,2
Macrophage Isolation and Culture Services at Creative Biolabs
At Creative Biolabs, we leverage over two decades of experience in biotechnology to deliver cutting-edge macrophage isolation and culture services. Our comprehensive services are tailored to meet the precise needs of academic and industrial clients exploring the biology of macrophages.
We provide a wide range of macrophages tailored to research needs, including:
Human monocyte-derived macrophages
Tissue macrophages are not easily obtained and differentiated blood-derived monocytes are largely used as surrogate models. Human blood monocytes are commonly used as target cells for in vitro biomaterial tolerance evaluation. Fully mature macrophages can be generated in the presence of cytokines. The culture of human monocyte-derived macrophages represents a tool to study macrophages.
Human alveolar macrophages
Alveolar macrophages are unique in lung development and function, as well as their lung-localized responses to infection and inflammation. Alveolar macrophages can be purified from bronchoalveolar fluid by adhesion to tissue culture plastic. If resected lung tissue is available, alveolar macrophages can be generated by mechanically disrupting the lung parenchyma, followed by adhesion-mediated purification.
Murine bone marrow-derived macrophages
Bone marrow-derived macrophages are primary macrophage cells derived from bone marrow cells in vitro in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage colony-stimulating factor (M-CSF). M-CSF is responsible for the proliferation and differentiation of committed myeloid progenitors into cells of the macrophage/monocyte lineage. Once differentiated, these macrophages are suitable for morphological, gene expression, and physiological studies.
Murine intestine macrophages
Macrophages play important roles in maintaining intestinal homeostasis via their ability to orchestrate responses to the normal microbiota as well as pathogens. One of the most important steps in beginning to understand the functions of these cells is the ability to effectively isolate them from the complex intestinal environment. Macrophages could be isolated from the mouse small and large intestine, and phenotypic characterization of these macrophages could be performed.
Murine alveolar macrophages
Alveolar macrophages are terminally differentiated, lung-resident macrophages of prenatal origin. Because the lung is exposed to pathogens and various environmental stimuli, infections and inflammation in the lung are common. Alveolar macrophages are an important component of host defense against invading microorganisms, and they play a critical role in the initiation and the resolution of inflammation in the lung.
Murine peritoneal macrophages
The peritoneal cavity provides an easily accessible site for harvesting moderate numbers of resident macrophages. Generally, macrophages isolated from the mouse peritoneal cavity will be mature quiescent macrophages. However, the yield is typically only about 0.5×106 macrophages per mouse. This yield can be increased by injecting eliciting agents into peritoneum several days before cell harvest.
Advanced Methods of Macrophage Isolation
Creative Biolabs is at the forefront of macrophage isolation, utilizing innovative techniques to ensure high purity, viability, and reproducibility. We employ state-of-the-art methodologies to ensure precise and efficient macrophage isolation from various biological sources.
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Methodologies
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Descriptions
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Types of Macrophages Available for Isolation
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Density Gradient Centrifugation
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A proven approach for separating macrophages based on their distinct density properties. This method is cost-effective and ideal for applications requiring large-scale cell preparation.
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Magnetic-Activated Cell Sorting (MACS)
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A high-throughput technique that uses magnetic beads conjugated with specific antibodies to isolate macrophages with exceptional purity and specificity.
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Fluorescence-Activated Cell Sorting (FACS)
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A powerful method that enables high-resolution isolation of macrophages based on fluorescence-labeled surface markers, allowing for subtype-specific separation and superior accuracy.
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Custom Macrophage Culture Systems
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Serum-free and defined media options: To minimize variability and support robust macrophage growth, we offer chemically defined and serum-free media formulations tailored to specific research needs.
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2D and 3D culture formats: For enhanced physiological relevance, we provide both traditional 2D monolayer cultures and innovative 3D culture systems, allowing for a more realistic study of macrophage behavior.
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Co-culture systems: To explore cellular interactions, we offer co-culture systems that enable macrophages to interact with various cell types, including T cells, cancer cells, and endothelial cells.
Related Products
We are your ideal partner for macrophage isolation and culture. In addition to our state-of-the-art methods and customizable solutions, we offer researchers a wide range of macrophage products from a variety of sources.
Creative Biolabs has developed a range of high-quality macrophage cell lines from different species and sources, including M0, M1, and M2 macrophages. You can click on the popular products below for product details.
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Cat.No
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Product Name
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Product Type
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MTS-0922-JF10
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Human Macrophages, Alveolar
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Human Macrophages
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MTS-0922-JF99
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Human M0 Macrophages, 1.5 x 10^6
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Human M0 Macrophages
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MTS-0922-JF52
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C57/129 Mouse Macrophages, Bone Marrow
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C57/129 Mouse Macrophages
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MTS-0922-JF7
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Human M2 Macrophages, Peripheral Blood, 10 x 10^6
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Human M2 Macrophages
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MTS-0922-JF34
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CD1 Mouse Macrophages
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CD1 Mouse Macrophages
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MTS-0922-JF13
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BALB/C Mouse Macrophages, Lung, 0.5 x 10^6
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Mouse Macrophages
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MTS-0922-JF16
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C57BL/6 Mouse Macrophages, Peritoneal Cavity, 6 x 10^6
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Mouse Macrophages
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MTS-0922-JF50
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C57BL/6 Mouse Macrophages (with LAT/LAB knockout ), Spleen
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Mouse Macrophages
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MT-0224-HM5
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Intraventricular Macrophages, Central Nervous System
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Mouse Macrophages
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MT-0224-HM22
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Sensory-nerve-associated Macrophages, Skin and Oral Mucosa
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Mouse Macrophages
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MTS-0922-JF84
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Porcine Macrophages, Bone Marrow, 6 x 10^6
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Porcine Macrophages
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Service Features
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Highly Customizable
Our services are highly customizable to support a wide range of experimental models and research objectives, including:
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Species specificity: Isolation from human, mouse, rat, and other species.
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Macrophage subtypes: Expertise in isolating tissue-resident macrophages and M1 or M2 macrophages.
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Tissue sources: Capable of isolating macrophages from blood, bone marrow, spleen, lung, and more.
Advanced Isolation Technologies
We employ state-of-the-art technologies, including:
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MACS: For highly specific and gentle cell separation.
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FACS: Enables isolation with extreme purity based on surface markers.
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Density gradient centrifugation: Efficient for bulk separation and enrichment of macrophage populations.
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Superior Culture Protocols
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High viability and purity: Guaranteed through optimized isolation protocols.
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Phenotype stability: Our culture systems preserve the functionality and polarization of macrophages.
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Serum-free media options: For researchers requiring xeno-free culture environments.
Comprehensive Analytical Support
We provide post-isolation characterization services, including:
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Flow cytometry: Quantitative analysis of macrophage purity and marker expression.
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qPCR and RNA-Seq: Profiling cytokine production and gene expression.
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Functional assays: Include phagocytosis, cytokine release, and antigen presentation assays.
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Workflow of Macrophage Isolation and Culture Services
Project Consultation→Sample Processing→Macrophage Isolation→Culture Setu→Quality Control and Characterization→Delivery and Support
Publications
Ping Yang et al. investigated the mechanism of metabolic interactions between tumor cells and macrophages in TME. In their experiments, bone marrow cells were isolated from the tibia and femur of mice and differentiated in the presence of M-CSF in RPMI-1640 growth medium. A co-culture system was then established using transwell insert plates, in which LLC cells were loaded into the upper insert plates and BMDM were inoculated in the lower compartments. After 2 days of co-culture, tumor-cultured macrophages were harvested and analyzed.
In this study, they explored the mechanisms of metabolic interactions between tumor cells and macrophages in TME through a co-culture system.
Fig. 2 The proposed model of CD36-mediated metabolic crosstalk between tumor cells and macrophages.3
Scientific Resources
Q & A
Q: Can you perform macrophage polarization to specific phenotypes (M1/M2)?
A: Yes, we offer comprehensive macrophage polarization services. We can generate M1 macrophages using IFN-γ and LPS stimulation, or M2 macrophages using IL-4/IL-13 treatment. Each polarization state is verified through expression analysis of characteristic markers and cytokine profiling. We can also customize polarization protocols to meet specific research requirements.
Q: What's your minimum sample size requirement, and how do you handle small tissue samples?
A: For peripheral blood, we typically require a minimum of 20 mL to ensure sufficient yield. For tissue samples, we can work with as little as 100 mg, using optimized protocols for small sample processing. We employ gentle isolation techniques and specialized culture conditions to maximize cell recovery from limited material. For precious samples, we can perform pilot runs with a portion of the sample to optimize conditions before processing the entire specimen.
Q: Are there any specific conditions I need to meet for sample submission?
A: Yes, we require that samples are collected under sterile conditions and shipped according to our provided guidelines. For blood samples, anticoagulants like EDTA are recommended. Tissue samples should be fresh or properly preserved. Our team will provide you with step-by-step instructions tailored to your sample type.
Q: What species do you support for macrophage isolation?
A: Our services cover multiple species, including human, mouse, rat, and other common research models. If you have unique species requirements, we encourage you to contact us with details so we can discuss feasibility and potential customization options.
Q: Can I provide specific instructions for my project?
A: Of course! We welcome specific instructions and will work closely with you to incorporate your requirements into the project. Whether it's using a specific isolation method, selecting markers for purity checks, or tailoring the culture environment, our team is highly flexible and committed to meeting your expectations.
References
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Ascoli, Bruna M., et al. "Attenuated inflammatory response of monocyte-derived macrophage from patients with BD: a preliminary report." International journal of bipolar disorders 7 (2019): 1-11.
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Under Open Access license CC BY 4.0, without modification.
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Yang, Ping, et al. "CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis." Nature Communications 13.1 (2022): 5782. Distributed under Open Access license CC BY 4.0, without modification.
