Macrophages are able to initiate a particular type of inflammatory response via the release of a certain pattern of cytokines. They play a major role as immunoregulatory cells. Creative Biolabs has built a highly experienced team of scientists and quality staff that have a long history in macrophage isolation and culture. Human monocyte, human alveolar, murine peritoneal cavity, murine bone marrow, murine lung, and murine adipose tissues are available for macrophage isolation and culture. Our seasoned scientists are pleased to share our expertise and experience with our global clients and facilitate their meaningful macrophage project development.
Fig.1 Workflow of monocyte isolation, and macrophage differentiation and polarization. (Ascoli, 2019)
Tissue macrophages are not easily obtained and differentiated blood-derived monocytes are largely used as surrogate models. Human blood monocytes are commonly used as target cells for in vitro biomaterial tolerance evaluation. Fully mature macrophages can be generated in the presence of cytokines. The culture of human monocyte-derived macrophages represents a tool to study macrophages
Alveolar macrophages are unique in lung development and function, as well as their lung-localized responses to infection and inflammation. Alveolar macrophages can be purified from bronchoalveolar fluid by adhesion to tissue culture plastic. If resected lung tissue is available, alveolar macrophages can be generated by mechanically disrupting the lung parenchyma, followed by adhesion-mediated purification.
Bone marrow-derived macrophages are primary macrophage cells derived from bone marrow cells in vitro in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage colony-stimulating factor (M-CSF). M-CSF is responsible for the proliferation and differentiation of committed myeloid progenitors into cells of the macrophage/monocyte lineage. Once differentiated, these macrophages are suitable for morphological, gene expression, and physiological studies.
Macrophages play important roles in maintaining intestinal homeostasis via their ability to orchestrate responses to the normal microbiota as well as pathogens. One of the most important steps in beginning to understand the functions of these cells is the ability to effectively isolate them from the complex intestinal environment. Macrophages could be isolated from the mouse small and large intestine, and phenotypic characterization of these macrophages could be performed.
Alveolar macrophages are terminally differentiated, lung-resident macrophages of prenatal origin. Because the lung is exposed to pathogens and various environmental stimuli, infections and inflammation in the lung are common. Alveolar macrophages are an important component of host defense against invading microorganisms, and they play a critical role in the initiation and the resolution of inflammation in the lung.
The peritoneal cavity provides an easily accessible site for harvesting moderate numbers of resident macrophages. Generally, macrophages isolated from the mouse peritoneal cavity will be mature quiescent macrophages. However, the yield is typically only about 0.5×106 macrophages per mouse. This yield can be increased by injecting eliciting agents into peritoneum several days before cell harvest.
Creative Biolabs combines deep, industry-leading expertise with an innovative Macrophage Therapeutics Development Platform to provide high-quality human/murine macrophage isolation and culture services. These isolated macrophages can be examined in vitro for their roles in phagocytosis, cytokine and mediator production, and other biological functions. Our R&D scientific team has accumulated extensive experience in comparing profiles of macrophages between controls and patients, which allows us to effectively determine the changes of the precursor number or the phenotype, with the eventual aim to help our clients develop cell therapies. For more information, please feel free to contact us and further discuss with our scientists.