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C57BL/6 Mouse Macrophages (with LAT/LAB knockout ), Spleen (MTS-0922-JF50)

Datasheet

Overview

Description

With linker for activation of T cells (LAT)/linker for activation of B cells (LAB) knockout, this product is isolated from the spleen of pathogen-free laboratory C57BL/6 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Spleen

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the difference between this product (JF50) and the LAB knockout (JF49)?

This product (JF50) is a DKO for both LAT and LAB. The other product (JF49) is a single KO for only LAB. You would use this DKO line if you want to completely eliminate signaling through either of these two key adaptor proteins, as they can sometimes have redundant functions.

What is the specific knockout in this cell line?

This is a double knockout (DKO) cell line. Both the Linker for Activation of T cells (LAT) and the Linker for Activation of B cells (LAB) have been knocked out. These are two closely related adaptor proteins involved in various immune receptor signaling cascades.

The cells are growing well and the DKO is solid
This LAT/LAB DKO line is a fantastic tool. We were seeing conflicting data with our single KO lines, and this DKO line proves the redundant function of LAT and LAB in our pathway.
The cells were clean, had high viability, and the fact that they are immortalized saves us so much time and effort
We confirmed the absence of both proteins. They are on the B6 background, which is perfect. My only note is that spleen-derived cells can be a bit trickier to culture, but following the recommended protocol from CB worked perfectly.
A very useful and specific cell line
The DKO of both adaptors gives us a 'clean-slate' background to study non-LAT/LAB signaling. The cells are CD11b positive and healthy. Thumbs up.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.