The efficacy of a macrophage-mediated inflammatory response depends on the coordinated expression of major factors. Their expression is finely regulated at both transcriptional and post-transcriptional levels. Several studies have provided strong evidence that microRNAs play key roles in modulating macrophage activation, polarization, tissue infiltration, and resolution of inflammation. As a leading specialist in macrophage development, Creative Biolabs excels at reprogramming macrophages by microRNA. Our professional technical scientists, comprehensive macrophage therapeutics development platform, and abundant experience make us a perfect partner to help our clients in macrophage reprogramming.

Macrophages Polarization and microRNAs

There are dynamic changes at gene loci associated with macrophage polarization in genome-wide studies. These changes resulted in the coordinated action of distinct signaling pathways and transcription factors. microRNAs are small (22-24 nt) non-coding RNA sequences that interact with the 3' UTRs of gene transcripts, suppressing their translation or driving their degradation. They are indispensable in regulating gene expression in both innate and adaptive immune cells.

Table 1 Key aspects of macrophage reprogramming by microRNAs

In conclusion, microRNAs play a multifaceted regulatory role in macrophage reprogramming, including influencing cellular metabolism, signaling pathways, and gene expression, thereby altering macrophage polarization status and function. This provides new ideas and possibilities for developing therapeutic strategies that target macrophages.

Reprogramming Macrophages by microRNA at Creative Biolabs

Creative Biolabs offers specialized services for reprogramming macrophages by microRNA. By utilizing microRNAs, our team of experts is able to efficiently regulate the gene expression of macrophages, thereby altering their functional state and promoting specific immune responses. Our reprogramming services include:

• microRNA screening and optimization: Based on the specific needs of the target study, we provide a range of functionally optimized microRNAs to ensure maximum reprogramming efficiency in macrophages.
• Reprogramming experiment design: For different research purposes (e.g. anti-tumor response, inflammation regulation, etc.), we will design personalized experimental protocols to achieve the best results.
• Functional testing and analysis: After reprogramming is completed, we will assess the functional changes of macrophages through various methods, including cytokine secretion, phagocytic capacity and other indicators, to ensure that the results meet expectations.
• Data analysis and reporting: Ultimately, we will provide detailed analysis of the experimental data and reports of the results to support subsequent studies.

Creative Biolabs is committed to providing high-quality and customized solutions to help you make more progress in your basic research. Please feel free to contact us to discuss your specific needs and we will be happy to assist you.

Related Products

Reprogramming macrophages by microRNAs has become a hot field in tumor microenvironment research. However, to deeply investigate the effects of microRNAs on macrophages, researchers need reliable experimental tools.

Creative Biolabs has introduced a breakthrough solution for macrophage research.

Service Features

Precision

Precise reprogramming of macrophages using specific microRNAs can effectively regulate their functions and phenotypes to meet the needs of different studies.

Efficient and Safe Reprogramming Technology

We use a proven method of combining miRNA mimics with modified mRNAs to avoid the potential risks of traditional viral vectors and provide a safer and more efficient reprogramming solution.

High-throughput Screening

We support high-throughput screening of a variety of microRNAs to help customers quickly find candidate molecules that meet their research needs.

Wide Applicability

Applicable to a wide range of disease models, we can customize specific miRNA combinations to meet different research needs.

Flexible Experiment Design and Optimization Services

Provide one-stop service, including miRNA screening, synthesis, transfection optimization and function verification to ensure the reliability of experimental results. We can adjust the culture system and transfection protocol for different experimental conditions.

Cutting-edge Research Support

We keep abreast of the latest scientific research results and combine advanced technology platforms to provide customers with high-quality services in line with international standards, while supporting personalized experimental design and data analysis.

Publications

Previous studies have identified specific subsets of microRNAs differentially expressed under distinct polarizing conditions, and investigated the effect of miRNA deregulation in macrophage polarization. A study identified 109 miRNAs differentially expressed in human and murine polarized macrophages, focusing in particular on miR-155, miR-181, and miR-451 in M1 macrophages, and miR-146a, miR-125a, and miR-145-5p in M2 macrophages. Subsequent studies confirmed the higher expression of miR-155 in M1-polarized macrophages and of miR-146a, miR-125b, and miR-127 in M2-polarizing conditions.

Fig.1 Pleiotropic role of miRNAs in the regulation of macrophage activation and polarization.^1,2

Scientific Resources

Learn More Macrophage Biology

Download Macrophage Polarization

Learn More Different Approaches to Regulating Polarization of Tumor-Associated Macrophages

Learn More Isolation and Identification of Macrophages

Q & A

Q: What is the exact process of microRNA reprogramming?

A: In our service, macrophages are first isolated and cultured. Next, we apply specific microRNA molecules, usually by means of transfection or viral vectors, to introduce the target microRNAs. This initiates a series of changes in intracellular signaling pathways, which in turn reprograms macrophage function. The entire process requires rigorous laboratory techniques and optimization to ensure that the targeted effects are achieved.

Q: How do I know if microRNA reprogramming is successful?

A: Successful microRNA reprogramming can be assessed by several metrics. We will perform functional tests on reprogrammed macrophages, including cell proliferation ability, cytokine secretion ability, and phagocytosis. In addition, we will monitor changes in specific gene and protein expression by methods such as qPCR and Western blot. Eventually, we will provide detailed experimental reports to help you understand the specific effects of reprogramming.

Q: Does microRNA reprogramming apply to all types of macrophages?

A: Yes, our microRNA reprogramming service can be applied to many types of macrophages, including M1-type and M2-type macrophages. The specific type to be applied will be selected based on your research goals and needs. We will adjust the microRNA selection and transfection protocols according to different macrophage types to ensure the best reprogramming results.

Q: How much does this reprogramming service cost for research?

A: The cost of the reprogramming macrophage service will be affected by a number of factors, including the complexity of the experiment, the amount of microRNA required, reagents and consumables. We will offer different service packages to meet the budget for different research needs. If you need, we can also discuss with you a personalized quotation plan based on the project size and specific needs.