Proliferative Capability Analysis Service by Proliferation Assay

The Significance of Macrophage Proliferative for Drug Development

Macrophages are a particular kind of innate immune cell that conducts unique inflammatory functions, including harm assessment, identification of pathogens, elimination, and restoration of wounds. The proliferation of macrophages is closely related to the occurrence and development of inflammatory diseases. Therefore, macrophage proliferation is an appealing therapeutic target for drug development. Creative Biolabs has established a well-equipped macrophage proliferative capability analysis platform. Together with our experienced research teams, we have more confidence in delivering the desirable proliferative capability analysis results for every customer to study macrophages.

Fig.1 Macrophage proliferation induced apoptotic cell.¹

Proliferative Capability Analysis Service by Proliferation Assay at Creative Biolabs: All-Inclusive! Sensitive! Dynamic!

Proliferative capability analysis service by proliferation assay from Creative Biolabs is a promising tool to analyze macrophage proliferative capability and is greatly useful for customers' meaningful macrophage projects. We target diverse examination markers to inclusively evaluate macrophage proliferative capability. Based on this, we provide a wide range of well-studied approaches (i.e. MTT, CCK-8, Luciferase assay) to perform this service. Simultaneously, we also develop several sensitive and dynamic technologies (i.e. RTCA) to meet your multiple demands. In addition, for macrophage isolating and culturing, we also deliver a service to help customers obtain high-quality macrophages for proliferation analysis. At Creative Biolabs, our standard assay format and simplistic setup will aid in achieving your diverse macrophage projects at a short turnaround.

Different analytical approaches have distinctive scopes of application. Here we provide several approaches to assist customers in choosing the appropriate approaches:

Macrophage metabolic activity assay: This method assesses macrophage proliferation capacity based on cellular metabolic capacity by reducing incubated substrates.
Macrophage DNA synthesis assay: This method measures cellular DNA synthesis to assess macrophage proliferation.
ATP content detection: ATP is a direct source of cellular energy, and its content is closely related to the cell cycle and cell status. This method is suitable for high-throughput cell proliferation ability assessment and screening.
Live cell fluorescent labeling: This method uses fluorescent dyes to label living cells and detect fluorescence density to evaluate cell proliferation ability.
Antigen detection associated with macrophage proliferative: Some antigens are only present in proliferating cells and are lacking in non-proliferating cells, so they are often used as markers of cell proliferation.
Dye exclusion: Living cells can reject certain dyes, while dead cells can be stained due to the destruction of membrane integrity. This method is suitable for the assessment of proliferation of a small number of cells.
Real-time cell analysis (RTCA): The microelectrode array is integrated at the bottom of each cell growth well of the cell culture plate to construct a real-time, dynamic, and quantitative cell impedance detection sensing system that tracks changes in cell morphology, proliferation, and differentiation.
Transplantation tumor animal model method: Cell proliferation is evaluated using parameters such as the size/volume and weight of the transplanted tumor, changes in the expression of proliferation-related molecular markers, and the area of necrotic areas within the transplanted tumor.

Frequently Asked Questions

Q1: How does the proliferation of macrophages occur?

A1: Evidence has showed that in vivo TH2 environments may reactivate and stimulate the proliferation of both resident and recruited macrophages. This means that proliferative ability is not lineage-dependent and that M2 macrophages do not have a particular precursor.

For more details about our proliferative capability analysis service by proliferation assay, please get in touch with us without hesitation.

Reference

Knuth, Anne-Kathrin, et al. "Apoptotic cells induce proliferation of peritoneal macrophages." International Journal of Molecular Sciences 22.5 (2021): 2230.