Macrophage proliferation is a tightly regulated process influenced by the tissue environment, immune modulators, and pathological cues. Unlike monocyte-derived macrophages, tissue-resident macrophages have the ability to self-renew locally, contributing to tissue homeostasis and regeneration. Accurate assessment of macrophage proliferation provides valuable insights into their functionality, plasticity, and responses to various stimuli or drug candidates.
Creative Biolabs offers a comprehensive macrophages proliferative capability analysis service utilizing state-of-the-art proliferation assay platforms. Designed for both basic and applied research, our service supports high-sensitivity quantification and characterization of macrophage proliferation under varied experimental conditions.
Macrophages are a particular kind of innate immune cell that conducts unique inflammatory functions, including harm assessment, identification of pathogens, elimination, and restoration of wounds. The proliferation of macrophages is closely related to the occurrence and development of inflammatory diseases. Therefore, macrophage proliferation is an appealing therapeutic target for drug development. Its key roles include:
Fig.1 Macrophage proliferation induced apoptotic cell.1,3
Creative Biolabs has established a well-equipped macrophage proliferative capability analysis platform. Together with our experienced research teams, we have more confidence in delivering the desirable proliferative capability analysis results for every customer to study macrophages.
Proliferative capability analysis service by proliferation assay from Creative Biolabs is a promising tool to analyze macrophage proliferative capability and is greatly useful for customers' meaningful macrophage projects. We target diverse examination markers to inclusively evaluate macrophage proliferative capability. Based on this, we provide a wide range of well-studied approaches (i.e. MTT, CCK-8, Luciferase assay) to perform this service. Simultaneously, we also develop several sensitive and dynamic technologies (i.e. RTCA) to meet your multiple demands. In addition, for macrophage isolating and culturing, we also deliver a service to help customers obtain high-quality macrophages for proliferation analysis. At Creative Biolabs, our standard assay format and simplistic setup will aid in achieving your diverse macrophage projects at a short turnaround.
Different analytical approaches have distinctive scopes of application. Here we provide several approaches to assist customers in choosing the appropriate approaches:
Each method is tailored based on the cell type (e.g., primary macrophages, RAW264.7, THP-1 derived macrophages) and experimental design. We provide a streamlined and customizable workflow to meet our clients' specific research objectives.
Table 1 Service process of macrophage proliferative capability analysis
Process | Descriptions |
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Experimental Design Consultation |
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Cell Culture & Differentiation |
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Proliferation Assay Execution |
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Data Acquisition & Analysis |
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Report Delivery |
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Building on our services, we have launched a series of specialized products to further support the needs of macrophage research. Our assay kits are designed to be simple and easy to use, dramatically reducing lab time and increasing lab efficiency, allowing researchers to focus more on their scientific explorations.
Our goal is to provide you with all-round support, both in terms of service and products, to help you take your scientific work further. Below are some of our popular products. You can click to view the details.
Cat.No | Product Name | Product Type |
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MTS-1022-JF1 | B129 Mouse Bone Marrow Monocytes, 1 x 10^7 cells | Mouse Monocytes |
MTS-0922-JF99 | Human M0 Macrophages, 1.5 x 10^6 | Human M0 Macrophages |
MTS-0922-JF4 | Human M2 Macrophages, Peripheral Blood (Age: 47), 2 x 10^6 | Human M2 Macrophages |
MTS-1022-JF6 | Human Cord Blood CD14+ Monocytes, Positive selected, 1 vial | Human Monocytes |
MTS-0922-JF7 | Human M2 Macrophages, Peripheral Blood, 10 x 10^6 | Human M2 Macrophages |
MTS-1123-HM6 | Macrophage Colony Stimulating Factor (MCSF) ELISA Kit, Colorimetric | Detection Kit |
MTS-1123-HM15 | Macrophage Chemokine Ligand 19 (CCL19) ELISA Kit, qPCR | Detection Kit |
MTS-1123-HM17 | Macrophage Chemokine Ligand 4 (CCL4) ELISA Kit, Colorimetric | Detection Kit |
MTS-1123-HM49 | Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, Colorimetric | Detection Kit |
MTS-1123-HM42 | Macrophage Receptor with Collagenous Structure ELISA Kit, Colorimetric | Detection Kit |
Investigators examined whether Osthole inhibited macrophage activation in vitro. Cells were preincubated with Osthole for 1 hour and then stimulated with IL-4 for 24 hours. Cell proliferation was measured by BrdU assay and colony formation. Osthole treatment attenuated IL-4-induced macrophage proliferation.
Fig. 2 Cell proliferation was measured by the BrdU assay and colony formation.2,3
Q: Is it possible to comparatively analyze macrophages from different treatment condition groups?
A: Of course it is possible. Our service supports multi-condition group assays, whether it is factor stimulation (e.g. LPS, IFN-γ), localized conditions (M1/M2), or drug experiments (e.g. chemotherapeutic agents, immunomodulators), with a multiple drug comparative analysis framework. We perform grouping based on the experimental design you provide and provide statistical significance analysis in the report final to ensure that the results are scientifically sound and sexually publishable.
Q: How long does the testing process take approximately? Can customers participate in the design or adjustment during the experiment?
A: The exact assay cycle depends on the selected assay platform, cell type and number of experimental groups. We welcome customers to participate in the design of the experiment to determine the source, treatment, sampling time point and other key parameters. If the experiment needs to adjust the protocol, we will also cooperate flexibly, provided that the adjustment will not affect data integrity and comparability.
Q: Is it possible to combine proliferation analysis with cytokine profiling?
A: Absolutely. We can integrate ELISA or multiplex cytokine assays to correlate proliferation status with functional outcomes.
Q: What types of macrophages can you offer for proliferation assays? Do you support customized sources?
A: Our support includes many types of macrophages, RAW264.7 cells, human-derived THP-1 cells, primary human macrophages derived from peripheral blood mononuclear cells (PBMC), and iPSC-derived macrophages. If you have specific needs, we also offer testing services for customer-sourced cells by simply providing the cells or specifying the isolation conditions.
References
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