Proliferative Capability Analysis Service by Proliferation Assay
Overview Our Service Related Products Service Features Publications Scientific Resources Q & A
Macrophage proliferation is a tightly regulated process influenced by the tissue environment, immune modulators, and pathological cues. Unlike monocyte-derived macrophages, tissue-resident macrophages have the ability to self-renew locally, contributing to tissue homeostasis and regeneration. Accurate assessment of macrophage proliferation provides valuable insights into their functionality, plasticity, and responses to various stimuli or drug candidates.
Creative Biolabs offers a comprehensive macrophages proliferative capability analysis service utilizing state-of-the-art proliferation assay platforms. Designed for both basic and applied research, our service supports high-sensitivity quantification and characterization of macrophage proliferation under varied experimental conditions.
Macrophage Proliferation
Macrophages are a particular kind of innate immune cell that conducts unique inflammatory functions, including harm assessment, identification of pathogens, elimination, and restoration of wounds. The proliferation of macrophages is closely related to the occurrence and development of inflammatory diseases. Therefore, macrophage proliferation is an appealing therapeutic target for drug development. Its key roles include:
-
Host defense: Rapid expansion during infection or injury
-
Tumor immunology: Tumor-associated macrophage (TAM) proliferation and polarization
-
Tissue repair: Resident macrophages proliferate during wound healing
-
Inflammatory regulation: Proliferation status reflects immune activation or resolution phases
Fig.1 Macrophage proliferation induced apoptotic cell.1,3
Creative Biolabs has established a well-equipped macrophage proliferative capability analysis platform. Together with our experienced research teams, we have more confidence in delivering the desirable proliferative capability analysis results for every customer to study macrophages.
Proliferative Capability Analysis Service by Proliferation Assay at Creative Biolabs: All-Inclusive! Sensitive! Dynamic!
Proliferative capability analysis service by proliferation assay from Creative Biolabs is a promising tool to analyze macrophage proliferative capability and is greatly useful for customers' meaningful macrophage projects. We target diverse examination markers to inclusively evaluate macrophage proliferative capability. Based on this, we provide a wide range of well-studied approaches (i.e. MTT, CCK-8, Luciferase assay) to perform this service. Simultaneously, we also develop several sensitive and dynamic technologies (i.e. RTCA) to meet your multiple demands. In addition, for macrophage isolating and culturing, we also deliver a service to help customers obtain high-quality macrophages for proliferation analysis. At Creative Biolabs, our standard assay format and simplistic setup will aid in achieving your diverse macrophage projects at a short turnaround.
Different analytical approaches have distinctive scopes of application. Here we provide several approaches to assist customers in choosing the appropriate approaches:
-
Macrophage metabolic activity assay: This method assesses macrophage proliferation capacity based on cellular metabolic capacity by reducing incubated substrates.
-
Macrophage DNA synthesis assay: This method measures cellular DNA synthesis to assess macrophage proliferation.
-
ATP content detection: ATP is a direct source of cellular energy, and its content is closely related to the cell cycle and cell status. This method is suitable for high-throughput cell proliferation ability assessment and screening.
-
Live cell fluorescent labeling: This method uses fluorescent dyes to label living cells and detect fluorescence density to evaluate cell proliferation ability.
-
Antigen detection associated with macrophage proliferative: Some antigens are only present in proliferating cells and are lacking in non-proliferating cells, so they are often used as markers of cell proliferation.
-
Dye exclusion: Living cells can reject certain dyes, while dead cells can be stained due to the destruction of membrane integrity. This method is suitable for the assessment of proliferation of a small number of cells.
-
Real-time cell analysis (RTCA): The microelectrode array is integrated at the bottom of each cell growth well of the cell culture plate to construct a real-time, dynamic, and quantitative cell impedance detection sensing system that tracks changes in cell morphology, proliferation, and differentiation.
-
Transplantation tumor animal model method: Cell proliferation is evaluated using parameters such as the size/volume and weight of the transplanted tumor, changes in the expression of proliferation-related molecular markers, and the area of necrotic areas within the transplanted tumor.
Each method is tailored based on the cell type (e.g., primary macrophages, RAW264.7, THP-1 derived macrophages) and experimental design. We provide a streamlined and customizable workflow to meet our clients' specific research objectives.
Table 1 Service process of macrophage proliferative capability analysis
|
Process
|
Descriptions
|
|
Experimental Design Consultation
|
-
Selection of optimal macrophage model and assay
-
Definition of controls, stimuli, and readouts
|
|
Cell Culture & Differentiation
|
-
Use of primary, immortalized, or iPSC-derived macrophages
-
Optional polarization to M1/M2 phenotypes
|
|
Proliferation Assay Execution
|
-
Incorporation of BrdU/EdU or CFSE labeling
-
Application of drug or immunological treatments as needed
|
|
Data Acquisition & Analysis
|
-
Quantitative readouts (absorbance, fluorescence, flow cytometry)
-
Advanced statistical comparison between conditions
|
|
Report Delivery
|
-
Detailed technical report including methodology, raw data, and interpretation
-
Recommendations for downstream experiments or therapeutic implications
|
Related Products
Building on our services, we have launched a series of specialized products to further support the needs of macrophage research. Our assay kits are designed to be simple and easy to use, dramatically reducing lab time and increasing lab efficiency, allowing researchers to focus more on their scientific explorations.
Our goal is to provide you with all-round support, both in terms of service and products, to help you take your scientific work further. Below are some of our popular products. You can click to view the details.
|
Cat.No
|
Product Name
|
Product Type
|
|
MTS-1022-JF1
|
B129 Mouse Bone Marrow Monocytes, 1 x 10^7 cells
|
Mouse Monocytes
|
|
MTS-0922-JF99
|
Human M0 Macrophages, 1.5 x 10^6
|
Human M0 Macrophages
|
|
MTS-0922-JF4
|
Human M2 Macrophages, Peripheral Blood (Age: 47), 2 x 10^6
|
Human M2 Macrophages
|
|
MTS-1022-JF6
|
Human Cord Blood CD14+ Monocytes, Positive selected, 1 vial
|
Human Monocytes
|
|
MTS-0922-JF7
|
Human M2 Macrophages, Peripheral Blood, 10 x 10^6
|
Human M2 Macrophages
|
|
MTS-1123-HM6
|
Macrophage Colony Stimulating Factor (MCSF) ELISA Kit, Colorimetric
|
Detection Kit
|
|
MTS-1123-HM15
|
Macrophage Chemokine Ligand 19 (CCL19) ELISA Kit, qPCR
|
Detection Kit
|
|
MTS-1123-HM17
|
Macrophage Chemokine Ligand 4 (CCL4) ELISA Kit, Colorimetric
|
Detection Kit
|
|
MTS-1123-HM49
|
Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, Colorimetric
|
Detection Kit
|
|
MTS-1123-HM42
|
Macrophage Receptor with Collagenous Structure ELISA Kit, Colorimetric
|
Detection Kit
|
Service Features
Customizable Protocols
Tailored design for specific macrophage lineages and research goals
Quantitative & Reproducible Results
Multiple assay formats with strict quality control
Advanced Analytical Tools
High-throughput readers, flow cytometry, and statistical data pipelines
Expert Biological Support
One-on-one consultation with scientists for experimental design
Comprehensive Reporting
Includes raw data, normalized results, and biological interpretation
Fast Delivery
Help researchers obtain timely experimental results
Publications
Investigators examined whether Osthole inhibited macrophage activation in vitro. Cells were preincubated with Osthole for 1 hour and then stimulated with IL-4 for 24 hours. Cell proliferation was measured by BrdU assay and colony formation. Osthole treatment attenuated IL-4-induced macrophage proliferation.
Fig. 2 Cell proliferation was measured by the BrdU assay and colony formation.2,3
Scientific Resources
Q & A
Q: Is it possible to comparatively analyze macrophages from different treatment condition groups?
A: Of course it is possible. Our service supports multi-condition group assays, whether it is factor stimulation (e.g. LPS, IFN-γ), localized conditions (M1/M2), or drug experiments (e.g. chemotherapeutic agents, immunomodulators), with a multiple drug comparative analysis framework. We perform grouping based on the experimental design you provide and provide statistical significance analysis in the report final to ensure that the results are scientifically sound and sexually publishable.
Q: How long does the testing process take approximately? Can customers participate in the design or adjustment during the experiment?
A: The exact assay cycle depends on the selected assay platform, cell type and number of experimental groups. We welcome customers to participate in the design of the experiment to determine the source, treatment, sampling time point and other key parameters. If the experiment needs to adjust the protocol, we will also cooperate flexibly, provided that the adjustment will not affect data integrity and comparability.
Q: Is it possible to combine proliferation analysis with cytokine profiling?
A: Absolutely. We can integrate ELISA or multiplex cytokine assays to correlate proliferation status with functional outcomes.
Q: What types of macrophages can you offer for proliferation assays? Do you support customized sources?
A: Our support includes many types of macrophages, RAW264.7 cells, human-derived THP-1 cells, primary human macrophages derived from peripheral blood mononuclear cells (PBMC), and iPSC-derived macrophages. If you have specific needs, we also offer testing services for customer-sourced cells by simply providing the cells or specifying the isolation conditions.
References
-
Knuth, Anne-Kathrin, et al. "Apoptotic cells induce proliferation of peritoneal macrophages." International Journal of Molecular Sciences 22.5 (2021): 2230. https://doi.org/10.3390/ijms22052230
-
Li, Ruyi, et al. "Osthole attenuates macrophage activation in experimental asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway." Frontiers in Pharmacology 12 (2021): 572463. https://doi.org/10.3389/fphar.2021.572463
-
Under Open Access license CC BY 4.0, without modification.
