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Human Cord Blood CD14+ Monocytes, Positive selected, 1 vial (MTS-1022-JF6)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.

Species

Human

Product Type

Immune cells

Specification

Source

Bone marrow

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How should we thaw, wash, and recover the cells to maximize viability and keep baseline activation controlled?

We recommend a rapid, controlled thaw followed by stepwise dilution into pre-warmed medium to reduce osmotic shock. To keep baselines consistent across plates, lock seeding density, medium composition, and the timing of cytokine additions or stimuli.

Can you support downstream differentiation/polarization and standardized validation if we want a turnkey workflow?

Yes. We can support monocyte-to-macrophage differentiation under defined cytokine conditions, optional polarization into defined activation states, and fit-for-purpose validation readouts. Typical deliverables can include phenotyping guidance or verification, cytokine profiling, and functional checks such as phagocytosis or stimulus-response assays, depending on your study.

If our post-thaw recovery or functional readout is lower than expected, what troubleshooting and resolution steps do you provide?

Most performance issues are driven by controllable variables rather than the cells themselves-thaw speed, wash intensity, density, recovery time, medium, or stimulation schedule. We troubleshoot quickly by reviewing your protocol and endpoints, then proposing targeted changes. Our goal is to get you back to decision-quality data with minimal repeats.

The shipment arrived well packaged, and the accompanying handling notes were practical-not generic
Post-thaw recovery was smooth, and we were able to transition directly into M-CSF differentiation with consistent adherence and morphology across replicates. What stood out was the responsive support: we asked about timing for stimulation and density optimization, and the team provided specific, experiment-oriented guidance that reduced our pilot iterations.
This cord-blood CD14+ monocyte vial gave us the consistency we needed for a short, high-pressure study window
The cells differentiated efficiently, and the macrophage-like phenotype was reproducible across plates. Communication was fast-questions about thawing, medium selection, and optional downstream validation were answered with clear rationale. The overall experience was professional and predictable, which is exactly what we need when coordinating with multiple teams and deadlines.
We ordered these cord blood CD14+ monocytes for an immunology course
The cells tolerated gentle handling and responded to cytokine priming in a way that made our functional readouts easier to interpret. The vendor's team also helped us refine our flow cytometry marker selection for early differentiation timepoints, which saved us time and reagents.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.