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Human PB CD14+ Monocytes (Age: 55), 1-2.5 x 10^7 cells (MTS-1022-JF46)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can we split the lot into multiple runs across different days?

It's possible, but we generally recommend minimizing repeated freeze-thaw exposure and batching your experiment to maintain consistency. If you must split across days, plan the split at the earliest step and keep processing parameters identical across runs (same recovery time, media, cytokines, and stimulation schedule). This reduces run-to-run drift and supports cleaner comparisons.

What are common pitfalls when differentiating monocytes from older donors, and how do we manage them?

Older-donor immune cells can show broader response variability, and some projects observe differences in baseline inflammatory tone or differentiation kinetics. The best mitigation is standardized handling and validation checkpoints-verify viability and baseline phenotype, then confirm differentiation markers/function before launching the full stimulation matrix.

Can you support assay selection if we want to benchmark macrophage function quickly?

Yes. For rapid benchmarking, we often suggest a compact panel that combines a phenotype confirmation step with one functional assay (e.g., phagocytosis or a defined stimulus cytokine response). This yields actionable information fast and can be scaled up if the results justify deeper profiling.

For labs that need a primary cell input that supports iterative study building, this is a practical and dependable choice
We often struggle when moving from pilot to validation because primary cell behavior can shift between lots or sizes. This Age 55 monocyte product maintained consistent handling and differentiation behavior, letting us transition smoothly. We ran a pilot stimulus panel first and then moved into validation experiments with confidence. The macrophages tolerated handling steps well and produced clean readouts.
Good balance of value and usability
Recovery was strong when following recommended handling practices, and differentiation was straightforward. We appreciated how predictable the macrophage yields were relative to our plating plan. In budget-conscious environments, this balance of scale and performance matters. Overall, the lot delivered consistent biology, reduced troubleshooting time, and helped us keep the project moving efficiently.
This lot performed like a reliable input rather than a variable to manage
We ran inflammatory challenge assays across multiple concentrations and observed clean, graded responses with good replicate consistency. Controls stayed quiet, and stimulated conditions produced expected marker changes. The macrophages were robust during media changes and sample collection, which reduced technical variability.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.