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Human PB CD14+ Monocytes (Age: 57), 2.5 x 10^7 cells (MTS-1022-JF49)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What are best practices for generating macrophages optimized for phagocytosis assays?

Phagocytosis performance depends on differentiation conditions, plating density, and the timing of functional assays relative to differentiation. We recommend optimizing a stable differentiation window, using a well-defined phagocytic substrate, and including both positive and negative assay controls. If your phagocytosis assay is mechanistic (receptor-mediated), we can also suggest phenotype checks to ensure relevant receptor expression.

Can you support custom stimulation panels (e.g., TLR agonists) and downstream cytokine profiling?

Yes. We can help you design a stimulation matrix that matches your biology and avoids common pitfalls like saturation dosing or timing mismatches. For cytokine profiling, we recommend consistent sample handling, appropriate normalization strategy, and inclusion of technical controls to ensure the dataset is robust and decision-ready.

What should we do if post-thaw recovery looks lower than expected?

First, confirm that thaw timing and DMSO removal were performed promptly and gently. Next, assess whether centrifugation settings or temperature exposure may have stressed the cells. We recommend checking viability, reviewing handling steps, and-if needed-running a small recovery optimization (media, rest time, seeding density). If you share your thaw notes, we can troubleshoot quickly and suggest corrective steps for your next run.

We were impressed by the post-thaw recovery and plating efficiency
The monocytes were easy to handle, and we did not need extra cleanup steps. After differentiation, the macrophages showed predictable marker profiles and robust responses to stimulation. The 2.5 × 10^7 size was convenient: enough for multiple experimental arms and repeats, but not so large that it creates waste.
A trustworthy input material for mechanistic immunology studies
We ran multiple stimuli and dose ranges to map response profiles, and the macrophages derived from this lot behaved consistently across plates. Controls remained stable while stimulated conditions produced clear, interpretable changes. That reliability allowed us to focus on biology rather than compensating for baseline drift.
This is a solid option for teams building complex in vitro models that require macrophages to behave predictably over time
Macrophages derived from this Age 57 lot integrated well into our co-culture system and showed controlled responses instead of chronic background activation. The cells tolerated the mechanical stresses of media changes and repeated sampling. Our readouts became cleaner and more reproducible than with prior sources.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.