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Human PB CD14+ Monocytes (Age: 29), 5 x 10^7 cells (MTS-1022-JF22)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you recommend splitting and staging the experiment to maintain cell state across a large workflow?

We recommend a single controlled thaw, rapid dilution, and then allocating cells immediately into pre-planned culture arms. Avoid holding large suspensions at room temperature for extended periods. Stagger plate setups and synchronize key steps (resting time, media change timing) to reduce drift.

Are there special considerations for centrifugation and washing at this scale?

Yes. Larger cell masses are more sensitive to shear and pellet compaction. Use gentle centrifugation settings, avoid tight pellets, and resuspend with care. Splitting into multiple tubes reduces mechanical stress and improves recovery.

Can I run macrophage differentiation plus functional assays and still reserve cells for repeat runs?

In many cases, yes. This format is designed to support both large primary workflows and contingency planning. We suggest reserving a defined percentage for repeat or validation assays rather than "leftover" cells that may age unpredictably.

For large experiments, the operational benefit of a high-count format is significant
We selected the 5 × 10^7 age-29 PB CD14+ monocytes because our design included multiple stimuli, multiple timepoints, and both functional and RNA endpoints. This format let us keep everything within one donor input, which improved the coherence of the dataset.
We used this batch to build a macrophage platform model and then execute a multi-condition functional panel
The supplier's communication was efficient and practical, including suggestions for tube-splitting and resuspension technique. When you're working at this scale, small handling details matter, and their guidance matched what we observed in the lab. Overall, it was a dependable, scalable solution.
We purchased these PB CD14+ monocytes for a cross-team project where several groups needed the same starting material
The 5 × 10^7 quantity made that coordination possible-each team received a controlled allocation from a single thaw, reducing variability. The cells were robust, responded predictably, and behaved consistently across different assay formats. For collaborative programs that require one standardized input across multiple assay modalities, this format is extremely useful.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.