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Human PB CD14+ Monocytes (Age: 50), 2.5 x 10^7 cells (MTS-1022-JF42)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these cells suitable for co-culture assays (e.g., with tumor cells, endothelial cells, or organoids)?

Yes, PB CD14+ monocytes are frequently used to build macrophage-based co-culture systems. Success depends on controlling differentiation timing, medium compatibility, and activation state to avoid unintended inflammation. If you share your co-culture partner cell type and readouts, we can advise on differentiation approach, seeding ratios, and validation checkpoints to make the model stable and reproducible.

Can you support custom characterization for macrophage differentiation outcomes (e.g., M-CSF vs GM-CSF)?

Yes. We can tailor characterization to your differentiation strategy, including marker panels that distinguish macrophage states and functional assays that validate the phenotype relevant to your program. This is particularly useful when comparing differentiation conditions head-to-head and you need data that clearly supports selection of the "best-fit" macrophage model.

How should we document these cells in our methods section for publications or reports?

We recommend documenting donor age, PB origin, CD14+ enrichment method (general description), cryopreserved status, cell dose, and key QC metrics (viability, phenotype confirmation, and contamination testing if performed). This level of detail typically satisfies reproducibility expectations while staying compliant with donor privacy constraints.

Great fit for inhibitor screening assays
We used macrophages derived from this lot in a small inhibitor screen where subtle shifts in cytokine profiles matter. The cells were stable in vehicle controls and showed measurable, dose-dependent changes with compounds. That's not always the case with primary myeloid cells. The differentiation process was straightforward, and the cells tolerated handling steps like media exchanges and repeated sampling.
If you're planning multi-day macrophage experiments, this lot handles the timeline well
Macrophages derived from this lot remained stable across our planned timepoints, and the phenotypes stayed consistent enough to support longitudinal comparisons. We ran multiple harvest points for RNA and supernatant and did not see unexpected variability. This made our analysis far cleaner and reduced the temptation to "over-normalize" data.
Straightforward to implement for busy labs
The recommended handling was clear, the recovery was strong, and the cell count was convenient for running multiple conditions. Our team was able to proceed directly into differentiation and functional assays without extra cleanup steps. The overall experience-ordering, receipt, documentation, and performance-felt consistent with a supplier that understands how primary immune cells are actually used in modern workflows.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.