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Monocyte Attachment Medium (MTS-1223-HM14)

Datasheet

Overview

Description

Creative Biolabs offers defined, animal-component free, and protein-free medium for efficient adherence selection of monocytes from freshly isolated human mononuclear cells.

Applications

Macrophage Culture

Product Type

Medium

Specification

Source

Human mononuclear cells

Size

250 mL

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is this medium specifically optimized to do in monocyte workflows?

This product is a defined, animal-component free, protein-free medium designed for efficient adherence selection of monocytes from freshly isolated human mononuclear cells, supporting monocyte enrichment via adhesion-based handling. It is supplied as 250 mL and positioned for macrophage culture workflows.

Is this medium appropriate for serum-free culture strategies?

The product is explicitly described as protein-free and animal-component free, which many teams use as a foundation for serum-controlled or serum-free approaches. Final culture strategy still depends on your differentiation cytokines and assay demands, so we recommend following the datasheet and aligning supplementation with your endpoint (e.g., resting macrophage vs activated phenotypes).

What are common pitfalls that reduce monocyte adherence yield?

The most common issues are inconsistent cell input quality (PBMC isolation variability), suboptimal adherence timing, overly aggressive washing, and temperature/pH drift. A simple way to improve reproducibility is to standardize your adherence window and washing force, and to record initial cell counts/viability at the start of each run.

In our hands, this attachment medium improved both yield and reproducibility
We used to lose a significant fraction of monocytes during the first wash and media change, but that loss dropped substantially after switching. The cells looked healthier and more evenly distributed, which made our activation assays easier to interpret. It also helped us run more comparable experiments across operators, since the attachment outcome became less dependent on technique.
The difference was immediate-attachment was more uniform and we had fewer "empty" regions on the plate
We used the Monocyte Attachment Medium for a co-culture setup where monocytes must adhere consistently before adding other cell types. The medium also supported gentle handling, which is important for primary myeloid cells.
It improved adherence without forcing harsh coatings or excessive manipulation
The cells spread evenly and maintained healthy morphology, which helped our microscopy and automated image analysis pipelines. We also saw fewer floating cells after media changes, suggesting better initial stabilization. Importantly, downstream responsiveness to stimuli was preserved, so we didn't feel we were trading function for adherence.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.