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Human PB CD14+ Monocytes (Age: 48), 1 x 10^7 cells (MTS-1022-JF40)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What phenotype drift should we expect during monocyte-to-macrophage differentiation, and how can we confirm identity?

During differentiation (e.g., M-CSF or GM-CSF based), monocytes typically downshift certain monocyte markers while increasing macrophage-associated markers and functional behaviors. We recommend confirming identity using a marker panel aligned to your biology (commonly including macrophage markers plus activation-state indicators) and validating function with a readout relevant to your mechanism-such as phagocytosis or cytokine secretion upon defined stimuli.

Do you offer guidance on polarization (M1/M2-like) or tissue-mimicking macrophage models?

Absolutely. Many projects require more than "generic macrophages." We can provide recommendations on polarization cues, timing, and media supplements to generate inflammatory, regulatory, or tissue-like macrophage phenotypes, and suggest validation assays to demonstrate the intended state. If you tell us your target pathway and assay endpoints, we can propose a practical differentiation/polarization workflow.

What shipping and storage conditions do you use, and what should we prepare on arrival?

Cells are shipped under cryogenic conditions to maintain viability and phenotype. Upon arrival, store immediately in vapor-phase liquid nitrogen if possible. We recommend preparing pre-warmed recovery media, gentle centrifugation settings, coated plates (if needed), and a clear schedule so the thaw and plating happen without delays.

The Age 48, 1 × 10^7 vial size was perfect for our method-development stage
We didn't want to commit to large lots before finalizing our macrophage differentiation timing and media conditions. The cells recovered well after thaw, and we achieved consistent adherence and morphology across plates. We also appreciated that the cell quantity matched our workflow without leftover waste, which is important when budgets are tight.
This is the kind of primary cell product that keeps projects moving
We purchased this lot specifically to run a cytokine secretion panel after TLR stimulation. The baseline secretion was low, which improved signal-to-noise, and the stimulus-response curves were nicely graded rather than "all-or-nothing." That made it easier to compare conditions and select concentrations for follow-on experiments.
If your team is building complex co-culture models and needs primary cells that behave predictably, this vial is a strong option
We use monocyte-derived macrophages in epithelial co-culture systems, where primary cell inconsistency can trigger false inflammatory phenotypes. This Age 48 monocyte lot differentiated smoothly and maintained stable behavior in co-culture, with less variability across wells than we typically see. The cells also tolerated the washing and media-change steps that often stress primary cells.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.