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Human PB CD14+ Monocytes (Age: 47), 2.5 x 10^7 cells (MTS-1022-JF39)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What QC metrics do you typically recommend before we run macrophage differentiation or functional assays?

For monocyte-to-macrophage workflows, we recommend a baseline QC set that includes viability (post-thaw), CD14 expression confirmation by flow cytometry, and a basic contamination screen. If you're performing cytokine profiling, phagocytosis, antigen presentation assays, or transcriptomics, adding endotoxin/mycoplasma testing and expanded immunophenotyping can materially improve interpretability. We can tailor QC depth based on whether the cells are used immediately or expanded/conditioned.

What is the best practice for thawing and initial recovery to protect monocyte responsiveness?

Monocytes can be particularly sensitive to osmotic shock and prolonged DMSO exposure. We recommend rapid thawing, gentle dropwise dilution into pre-warmed recovery medium, and prompt centrifugation to remove cryoprotectant. Avoid harsh pipetting and minimize time at room temperature. After recovery, allow a short rest period before stimulation or differentiation to stabilize signaling pathways and reduce artifactual activation.

We used the Age 47, 2.5 × 10^7 Human PB CD14+ monocytes as the starting population for a macrophage polarization panel
After thaw and recovery, the cells attached evenly, expanded to our planned density, and differentiated with minimal spontaneous activation. The IL-4/IL-13 arm showed strong CD206 induction, while the LPS/IFN-γ arm produced a crisp cytokine shift without the "noisy" baseline we sometimes see from other vendors.
These CD14+ monocytes performed exceptionally well in functional assays
We ran fluorescent bead phagocytosis and oxidative burst readouts at multiple timepoints post-differentiation and saw consistent uptake kinetics across wells. The key win was reproducibility: plate-to-plate variability decreased noticeably, which saved us repeats and reagent cost. Shipping and packaging were professional, and the vial format fit our batch scheduling.
Our group differentiates monocytes into monocyte-derived dendritic cells for antigen presentation studies
Surface marker development after cytokine induction was strong and consistent, and we obtained clear T-cell activation signals without extensive optimization. Importantly, the cells tolerated the handling steps required for peptide pulsing and co-culture, which often expose weaknesses in cryopreserved primary lots.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.