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Human PB CD14+ Monocytes (Age: 31), Negative selected, 1 x 10^7 cells (MTS-1022-JF25)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Is 1 × 10^7 cells enough for multi-condition screening?

For many workflows, yes-especially if you plan a focused panel (e.g., 2-4 polarization conditions plus controls) or scale assays into 96-well formats. We recommend mapping your cell budget backward from endpoints: imaging often needs fewer cells than secretome profiling; RNA-seq typically demands higher per-condition input. If you share your plate map, we can help optimize densities without compromising phenotype.

Will negative selection affect differentiation speed or attachment?

Differentiation outcomes depend more on recovery quality, cytokine regimen, and plating density than on selection label. The practical tip: after thaw, allow a short recovery period and remove residual cryoprotectant efficiently (gentle dilution and wash). Then seed consistently and avoid "over-handling" in the first 12-24 hours.

How do you recommend storing and using partial vials?

We don't recommend re-freezing or "partial vial" storage because temperature cycling is a major viability killer and can skew functional responses. Plan your experiment so a full vial is used in a single thaw event. If you need smaller lots routinely, this 1 × 10^7 format is designed for that purpose.

We selected the negative-selected Age 31 PB CD14+ monocytes specifically because our assays are highly sensitive to baseline activation
After a careful thaw and gentle wash, the cells behaved predictably and showed a clean baseline in our phospho-signaling panel. The 1 × 10^7 size matched our screening needs without wasting material. We also appreciated the clear shipping and LN2 storage guidance, which helped standardize handling across operators.
We don't always need a huge monocyte input-often it's a pilot study or method development
This vial size was perfect. Differentiation to macrophages was smooth, and the cells responded well to polarization cues. The negative-selection aspect was a plus because we're comparing subtle changes in cytokine profiles.
In culture, the cells differentiated reliably, and our polarization markers were consistent across repeats
The best way I can describe it: the cells were responsive when we stimulated them, but they didn't look stressed at baseline. That's exactly what we need for TLR comparisons. The 1 × 10^7 vial size makes budgeting easy, and the supplier's handling notes aligned with what our team considers best practice.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.