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Human PB Monocytes, Positive selected, 1 x 10^7 cells (MTS-1022-JF78)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.

These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What exactly is included in this product unit?

The listing specifies 1×10^7 cells per vial, supplied in a cryopreserved format-ideal for standardized day-0 seeding, replicate planning, and repeatable assay startup.

How should I store and manage the vial to maximize recovery?

Shipments arrive on dry ice; transfer to liquid nitrogen (-150°C to -190°C) immediately upon receipt, and avoid repeated freezing and thawing to maintain viability and functional responsiveness.

Why is positive selection beneficial for PB monocytes in some workflows?

Positive selection uses CD14-directed immunomagnetic capture, often favored when you want a strong CD14 identity for consistent phenotyping, downstream differentiation, or QC alignment with pre-defined CD14 gating strategies.

This positive-selected monocyte lot helped us tighten our macrophage assay workflow by reducing input heterogeneity
We observed clean population profiles and strong reproducibility in functional assays after stimulation. The cells differentiated consistently and produced interpretable phenotype shifts under polarization conditions.
We used this positive-selected monocyte product to generate macrophages for a comparative panel study
The input consistency translated into more uniform differentiation and less variability between replicates. The cells were easy to count and distribute, and their behavior in culture was stable. We also found the supplier communication reliable-quick confirmations, clear shipment tracking, and documentation ready for filing.
Our lab uses monocytes for assays
The 1 × 10^7 format was practical for multiple plates while still manageable for scheduling. Post-thaw handling was smooth, with limited aggregation. We were able to move directly into experiments without excessive recovery steps.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.