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Human PB CD14+ Monocytes (Age: 44), 1 x 10^7 cells (MTS-1022-JF37)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Is 1 × 10^7 cells/vial enough for a pilot macrophage polarization study?

It typically supports baseline differentiation plus a focused set of stimulation conditions (e.g., M0 vs. one polarization arm with technical replicates), or a smaller co-culture proof-of-concept. It's also a good choice if you want to validate your protocol and readouts before scaling to 2.5 × 10^7 or 5 × 10^7 formats.

Can these cells be used for phagocytosis assays after differentiation?

Yes. Many teams differentiate CD14+ monocytes into macrophages and then assess functional endpoints such as phagocytosis. To improve interpretability, we recommend including a consistent recovery period post-thaw, standardizing differentiation days, and running a baseline phenotype check before the functional assay. This reduces the risk that a transient post-thaw stress state influences uptake behavior or cytokine background.

What precautions should we take to prevent viability loss during processing?

Keep processing time tight, handle gently, avoid harsh centrifugation, and never refreeze. Because the vial size is optimized for single-run use, designing your workflow so the vial is consumed in one controlled experiment generally yields the best recovery and data consistency. Follow the receiving guidance: immediate LN2 storage upon arrival and minimal temperature fluctuation.

This smaller format worked well for rapid feasibility experiments
The vial size was perfect for side-by-side testing without wasting cells. Thawing and plating were straightforward, and we observed predictable differentiation with clear morphological changes. Documentation was easy to file for our lab records, and overall performance was strong for a pilot format.
This Age 44 monocyte vial performed well and produced consistent differentiation outcomes under our standard SOP
In shared facilities, cell quality issues can spiral into weeks of troubleshooting. The smaller size is ideal for groups who want to learn the workflow and generate preliminary data without overcommitting resources. We also liked that the product is explicitly cryopreserved.
We ran a controlled comparison of stimuli and needed a clean monocyte input
Viability and plating were acceptable, and the derived macrophages produced consistent readouts across replicates. The ordering process was smooth and the datasheet supported our internal review.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.