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Human PB CD14+ Monocytes (Age: 30), Positive selected, 1 vial (MTS-1022-JF23)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can you recommend a practical "go/no-go" check right after thaw?

A quick viability assessment and a small flow check for CD14 enrichment are common. Many customers also seed a small fraction to confirm early adherence and morphology before committing the full vial to multi-day differentiation.

How do you help ensure consistent macrophage differentiation outcomes?

We provide handling guidance that focuses on seeding density, rest timing, and media schedule-variables that strongly influence differentiation. If you share your endpoints (marker panels, functional readouts), we can suggest adjustments that reduce variance.

What if my assay involves stimulation and I'm concerned about baseline activation?

We recommend controlled recovery, minimized shear, and a consistent rest period. Avoid overly aggressive washes and temperature fluctuations. Establishing a stable baseline before stimulation improves interpretability.

This product performed exactly as we needed for a routine macrophage platform
The enrichment quality supported clean flow phenotyping after differentiation, and our assays showed stable baseline behavior before stimulation. Shipping and packaging were reliable, and the documentation was clear enough for internal review. The technical team also answered our questions about splitting the vial for imaging vs functional assays, and their suggestions helped us keep both arms aligned.
It's a solid RUO product for teams building repeatable macrophage experiments without spending weeks optimizing starting material
We ordered this vial for a pilot study and ended up extending the work because the cells behaved consistently. Recovery was strong, and the cells transitioned into macrophage-like states with predictable kinetics. We then ran a stimulation panel and observed clear, interpretable responses with controlled baseline noise. The 1-vial format also reduced waste and simplified scheduling.
We would recommend this format for labs aiming to reduce variability and streamline macrophage-based assays
The vial yielded enough cells to run multiple conditions with appropriate replicates while keeping the donor input constant. Post-thaw recovery was solid and the cells were responsive to defined stimuli after a standardized rest. The documentation was straightforward, and the handling notes helped prevent common issues like clumping and baseline drift. The overall experience was professional and consistent with what we need for repeatable research workflows.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.