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Human PB Pan Monocytes (Age: 46), Negative selected, 5 x 10^7 cells (MTS-1022-JF84)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. Monocytes provide an ideal model for the study of macrophage biology and mechanisms.
These pan monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you help ensure run-to-run reproducibility if I'm doing a multi-week study?

Same lot where possible, consistent thaw density, and standardized differentiation timing. We can support this by coordinating multi-vial supply from the same lot and recommending a simple incoming QC workflow (post-thaw viability + CD14 enrichment check) so each run begins with documented comparability.

Are these cells appropriate if I need monocytes that behave "resting" at baseline?

Negative selection is often preferred for baseline-sensitive assays because it reduces direct antibody/bead engagement of monocyte surface antigens. After thaw, you can further stabilize baseline by using a short recovery period, controlled serum conditions, and avoiding harsh RBC lysis or over-centrifugation. If your endpoint is signaling (NF-κB, inflammasome, phospho-flow), we can suggest practical steps to reduce artifactual activation.

Can you support downstream services if I want to convert these monocytes into macrophage models?

Yes. Many teams pair primary monocytes with downstream macrophage differentiation and functional profiling. If you want to reduce internal workload, we can help you map a clean workflow-from thaw parameters, to differentiation conditions, to phenotype/function readouts-so you receive data that is study-ready rather than just a vial of cells.

Delivery conditions were reliable and the labeling was clear, which helps when samples enter shared facility workflows
We used these monocytes for antibody-dependent cellular phagocytosis (ADCP)-style experiments after differentiation. The assay performance was excellent: clean negative controls, strong stimulation responses, and consistent donor behavior across the panel. We appreciated the negative selection approach because it minimized interference in Fc-mediated readouts.
Our lab uses monocytes for co-culture systems with tumor cells
This batch delivered stable adherence and morphology, and the macrophages maintained good viability in co-culture conditions over several days. Importantly, we saw consistent cytokine profiles across replicates, which helped us detect tumor-induced shifts with higher confidence. The cells also performed well in phagocytosis assays and did not show unexpected variability.
We would reorder for omics-focused projects where clean biology matters
These monocytes behaved well in culture, with consistent differentiation kinetics and strong RNA quality in our preliminary QC. Another advantage was the practical support-Creative Biolabs answered questions about recommended recovery time and media composition quickly and with actionable detail.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.