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Human PB Monocytes (Age: 48), Negative selected, 1 x 10^7 cells (MTS-1022-JF76)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the key advantage of using an age-defined donor (Age: 48) for PB monocyte studies?

Age can influence baseline immune tone and monocyte functional responsiveness. Using an age-defined donor strengthens experimental control-particularly in comparative studies (e.g., age-matched controls, immune aging hypotheses, or consistency across multi-batch campaigns).

How are these monocytes isolated, and why does that matter?

They are purified by immunomagnetic negative selection (removal of other cell types). This is often preferred when you want enriched monocytes without direct target capture that could bias surface-marker behavior before stimulation.

What is the vial content and format-can I use it for standardized plate layouts?

The listing specifies 1×10^7 cells per vial, and the product is cryopreserved, which is suitable for standardized seeding plans across assay plates and repeatable differentiation setups.

This age-defined monocyte lot supported our internal reference panel for innate immune profiling
The negative-selection approach was beneficial for maintaining low baseline activation, which improved the interpretability of our early time-point readouts. Recovery and handling were consistent, with minimal clumping and good seeding behavior. Differentiated macrophages displayed stable marker expression and strong response to our stimulation conditions.
We've had challenges in the past with monocyte lots that arrive stressed or partially activated
The cells were suitable for both short-term stimulation assays and longer differentiation protocols. Documentation was easy to integrate into our electronic lab notebook system, and shipment tracking updates were timely.
Our team needed a defined donor age monocyte product to support a comparative dataset across different immune models
We appreciated that the cells behaved calmly before stimulation-minimal spontaneous cytokine release and stable baseline marker expression. After activation, signals were strong and reproducible.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.