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Human PB CD14+ Monocytes (Age: 0), Positive selected, 2.5 x 10^7 cells (MTS-1022-JF9)

Overview

Description
Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.

These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Species
Human
Product Type
Immune cells

Specification

Source
(Peripheral blood) PB
Isolation Method
Positive selection
Format
Cryopreserved
Disease State
Normal
Donor Attributes
Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)
Quality Control
Cells are negative for mycoplasma, bacteria, fungi and yeast.
Shipping Info
Dry ice
Size
2.5 x 10^7 cells
Handling Notes
Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.
Notes
Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer
This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.
FAQs Customer Reviews Related Products

We plan to generate macrophages for infection/immunology assays-what workflow do you recommend?

Start with a controlled recovery after thaw, then differentiate under defined cytokine conditions tailored to your endpoint (phagocytosis, cytokine secretion, antigen presentation, or transcriptomic profiling). For infection models, it's important to standardize multiplicity of infection timing relative to differentiation stage, and to confirm baseline viability and adherence before challenge.

Why choose positive selection for neonatal PB monocytes, and what are the trade-offs?

Positive selection offers strong enrichment for CD14+ cells and typically supports cleaner starting populations for differentiation and functional assays. The trade-off is that selection steps can introduce handling and may affect certain activation-sensitive readouts if not standardized. We mitigate this by providing handling guidance and recommending a consistent rest period post-thaw before stimulation.

Can you help us scale from exploratory work to repeated campaigns?

Yes. For programs that require repeated runs, we recommend planning for lot strategy and assay standardization early: define acceptance criteria (post-thaw recovery, baseline phenotype), lock down differentiation conditions, and use reference controls across campaigns. We can support this with consistent supply planning, optional functional validation services, and standardized reporting so your team can scale without reinventing the workflow each time.

  • If your study depends on early-life myeloid cells, this product is worth it
    These age 0 CD14+ monocytes were a great fit for our biomaterials co-culture project, where monocyte behavior can be hypersensitive to handling. The thaw was clean with minimal aggregates, and adhesion in macrophage differentiation conditions was uniform. We measured CD14 enrichment and saw a tight distribution, which improved our readouts in surface marker panels and secretome profiling.
  • From ordering to execution, this was a smooth experience
    We specifically needed a positive-selected CD14+ prep with sufficient cell numbers for multiple assay arms, and 2.5×10^7 cells per vial was a practical format. We used the cells for phagocytosis assays and oxidative burst measurements; both readouts were stable across plates, suggesting good functional integrity.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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