Start with a controlled recovery after thaw, then differentiate under defined cytokine conditions tailored to your endpoint (phagocytosis, cytokine secretion, antigen presentation, or transcriptomic profiling). For infection models, it's important to standardize multiplicity of infection timing relative to differentiation stage, and to confirm baseline viability and adherence before challenge.
Positive selection offers strong enrichment for CD14+ cells and typically supports cleaner starting populations for differentiation and functional assays. The trade-off is that selection steps can introduce handling and may affect certain activation-sensitive readouts if not standardized. We mitigate this by providing handling guidance and recommending a consistent rest period post-thaw before stimulation.
Yes. For programs that require repeated runs, we recommend planning for lot strategy and assay standardization early: define acceptance criteria (post-thaw recovery, baseline phenotype), lock down differentiation conditions, and use reference controls across campaigns. We can support this with consistent supply planning, optional functional validation services, and standardized reporting so your team can scale without reinventing the workflow each time.
For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.