HL-60 cells serve as an invaluable tool for advancing our understanding of leukemia, hematopoiesis, and various other aspects of cell biology and disease pathology. Creative Biolabs has developed HL-60-based macrophage models, providing a valuable experimental system for elucidating the biology of macrophages and their role in health and disease, and a versatile platform for researchers to conduct mechanistic studies, drug screening assays, and translational research work.

HL-60 Cells

HL-60 cells are a type of human promyelocytic leukemia cell line commonly used in biomedical research, particularly in studies related to hematology, immunology, and cancer. These cells were originally isolated from the peripheral blood of a patient with acute promyelocytic leukemia (APL) in 1977.

HL-60-based Macrophage Model Development Service at Creative Biolabs

Creative Biolabs provides services for the development of HL-60-based macrophage models, including cell culture, cell differentiation, immunological assays, and data analysis. Creative Biolabs has experience in developing and validating such models to ensure their reliability and relevance for research or drug discovery purposes.

If you have any needs other than HL-60-based macrophage model development services, feel free to contact us our scientists!

Display Results

HL60 cells treated with different differentiation agents
Fig.1 HL60 cells treated with different differentiation agents.1	The cells shown on the left were treated with silica MP for 3 days. The cells were then fixed and subjected to immunocytochemical CD11b staining, followed by DAPI staining, and the CD11b expression level of HL60 cells treated with PMA and DMSO was evaluated.

Frequently Asked Questions

Q1: Why choose the HL-60-based macrophage model?

A1: HL-60-based macrophage models offer several advantages in studying macrophage biology and related fields:

• Unlike primary macrophages, which can exhibit donor-to-donor variability, HL-60 cells offer a standardized and uniform cell population, simplifying experimental comparisons and data interpretation.
• HL-60 cells can be cultured in large quantities, ensuring an ample supply of macrophages for experiments requiring large cell numbers or prolonged culture periods.
• By treating HL-60 cells with differentiation agents such as phorbol esters (e.g., phorbol 12-myristate 13-acetate, PMA) or specific cytokines (e.g., granulocyte-macrophage colony-stimulating factor, GM-CSF), researchers can generate mature macrophages with defined phenotypic and functional characteristics.
• HL-60 cells are amenable to genetic manipulation and molecular interventions, allowing researchers to modulate gene expression or signaling pathways to investigate the molecular mechanisms underlying macrophage biology, inflammation, immune responses, and disease pathogenesis.
• HL-60-derived macrophages can be used in a wide range of experimental assays commonly employed to study macrophage functions, including phagocytosis assays, cytokine secretion assays, chemotaxis assays, and receptor signaling studies.