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Human M1 Macrophages, 1.5 x 10^6 (MTS-0922-JF98)

Datasheet

Overview

Description

This product is isolated from human peripheral blood. Donors are screened for hepatitis B, hepatitis C, HIV-1 and HIV-2. 1.5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Human

Product Type

Human M1 Macrophages

Specification

Cell Source

Peripheral blood

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How stable is the M1 phenotype over time in culture, especially in multi-day experiments?

The M1 phenotype remains stable for several days under recommended culture conditions. With appropriate medium, careful handling, and controlled stimulation, the cells continue to display elevated inflammatory markers and functional responses over typical experimental windows of 2-5 days. For extended time courses, we advise regular monitoring and, if needed, re-stimulation to maintain the desired activation level.

Do these M1 macrophages respond to a broad panel of TLR agonists?

They respond robustly to multiple TLR ligands, including LPS (TLR4), poly(I:C) (TLR3), and other commonly used agonists. These stimuli induce strong cytokine and chemokine production, as well as upregulation of activation markers.

What precautions should I take during thawing and initial seeding to preserve their pro-inflammatory phenotype?

Rapid thawing in a 37°C water bath, immediate dilution into pre-warmed culture medium, and gentle centrifugation are key to maintaining high viability. We recommend seeding at the suggested density and allowing sufficient recovery time (typically overnight) before applying experimental treatments. Avoid excessive mechanical stress and extreme temperature changes, as these can impact both viability and phenotype stability.

These human M1 macrophages have become our go-to model for testing anti-inflammatory candidates
The cytokine responses to LPS and other TLR agonists are strong and reproducible, and the cells perform very well in 96-well screening formats. Using a ready-to-use M1 population has simplified our workflow tremendously.
This product has helped us generate high-quality data for several internal projects
We were impressed by the clear expression of activation markers and the robust secretion of pro-inflammatory cytokines. The phenotype remained stable for several days, which allowed us to run longer kinetic experiments without losing signal quality.
The thawing protocol was straightforward, and the cells recovered quickly
They adhered firmly, displayed a classic M1 morphology, and gave very consistent ELISA and qPCR readouts. Technical support also provided helpful tips for optimizing our stimulation conditions.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.