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General Macrophage Medium (MTS-1223-HM1)

Datasheet

Overview

Description

Creative Biolabs offers customized, serum-free and xeno-free medium for generation of macrophages from fresh monocytes. Our macrophage medium does not include cytokines.

Applications

Cell Culture

Product Type

Medium

Specification

Component

Basal Medium; SupplementMix

Size

250 mL

Handling Notes

Do not freeze the Basal Medium. Do not freeze the complete medium.

Storage

Basal Medium at 4-8°C in the dark; SupplementMix at -20°C immediately after arrival.

Notes

This product needs additional supplementation with M-CSF / GM-CSF.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Is this General Macrophage Medium suitable for both human and rodent macrophages, including primary cells?

Yes. The medium is formulated to support a broad range of macrophage types, including primary human and rodent macrophages as well as monocyte-derived macrophage cultures. It provides balanced nutrients, growth factors, and serum to sustain viability, adherence, and function in typical in vitro conditions. Many users standardize on this medium to reduce variability when working with multiple species in parallel.

Does the medium contain serum, and can I adjust the serum level if needed?

The standard formulation is supplied as a complete medium with optimized serum content to support robust macrophage growth and function. If your protocol requires alternative serum levels or specific serum sources, please contact us for guidance.

Does the medium contain antibiotics, and is an antibiotic-free version available?

The standard version includes antibiotics for routine culture convenience. However, we understand that some assays or downstream applications (e.g., microbiome-related work, sensitive omics analyses) require antibiotic-free conditions. An antibiotic-free formulation is available upon request, allowing you to maintain full control over experimental variables while preserving the same basal composition.

Switching to this General Macrophage Medium significantly improved the consistency of our macrophage cultures
Both human monocyte-derived macrophages and rat primary cells showed better survival, adherence, and functional responses compared with our previous in-house medium. It has become a staple in our lab.
We use the antibiotic-free version for experiments involving live bacteria
The basal formulation still supports excellent macrophage viability and function, and our contamination rate actually decreased because we refined our aseptic techniques. It's a very solid, flexible medium.
Our team can now focus more on the biology and less on solving culture issues, which is a big plus
Overall, this is a high-quality macrophage medium that has reduced our need for extensive in-house optimization.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.