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BALBC Mouse Macrophages (with STAT6 knockout), Bone Marrow (MTS-0922-JF57)

Datasheet

Overview

Description

With STAT6 knockout, this product is isolated from the bone marrow of pathogen-free laboratory BALB/C mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

BALBC Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What changes should I expect compared to wild-type BALB/c macrophages?

Compared with wild-type macrophages, these STAT6 KO cells exhibit significantly reduced expression of anti-inflammatory genes, impaired alternative activation morphology, and diminished surface marker changes associated with M2 polarization. Many researchers use them alongside wild-type cells to quantify the extent of STAT6 dependency in their M2 models.

What culture conditions do you recommend to maintain viability and macrophage functionality after thawing?

We recommend culturing the cells in RPMI-1640 supplemented with 10% heat-inactivated FBS, penicillin/streptomycin, and M-CSF to support macrophage maintenance. Upon thawing, cells generally reattach and spread within 24 hours if seeded at appropriate densities. Maintaining consistent serum sources and cytokine supplements helps ensure reproducible responses to polarization assays.

Do you offer supporting reagents or experimental guidance for setting up STAT6-dependent assays?

Our technical support team can provide recommendations for IL-4/IL-13 stimulation conditions, suggested marker panels for flow cytometry, or gene panels for qPCR.

The cells revived quickly and behaved exactly as expected for STAT6-deficient macrophages
Our lab conducted RNA-seq, and the transcriptional signature aligned perfectly with known STAT6 biology. Having a stable knockout model saved us significant time.
We used these BALB/c STAT6 KO macrophages for IL-4-driven M2 studies and the knockout phenotype was striking
The cells showed minimal induction of Arg1 and other canonical M2 genes, which helped us validate our pathway inhibitors. The morphology and viability were excellent post-thaw.
This level of detail really boosted our confidence in the model
We compared wild-type and STAT6 KO macrophages to study IL-13 responses. The difference in gene activation was very clear and reproducible across replicates. The supplier's thawing instructions were easy to follow.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.