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C57BL/6 Mouse Macrophages (with PKR KO knockout), Bone Marrow (MTS-0922-JF54)

Datasheet

Overview

Description

With protein kinase R (PKR) knockout, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these PKR KO macrophages suitable for viral infection models and innate immunity signaling studies in vitro?

Yes. They are highly suitable for dissecting PKR-dependent antiviral signaling pathways, including changes in type I interferon responses and downstream ISG induction. Many customers use them side-by-side with wild-type macrophages to distinguish PKR-specific effects on viral replication or immune evasion strategies. Because they are derived from the widely used C57BL/6 background, they also integrate well with existing mouse models and datasets.

What is the typical cell viability and recovery rate after thawing?

Under standard conditions, users typically achieve more than 90% viability right after thawing, provided that our protocol is followed closely. We supply step-by-step thawing and recovery instructions optimized specifically for these PKR KO macrophages. If you encounter any difficulty during the first recovery, our technical support team can help troubleshoot and suggest adjustments based on your culture system.

Can I order larger lots or customized batch sizes if I plan to run high-throughput antiviral screening with these cells?

Absolutely, we routinely support projects that require larger or repeated lots of the same PKR KO line. For high-throughput or long-term studies, we can arrange customized production runs to match your required cell number, format, and delivery schedule.

Reliable performance across multiple experiments
We have ordered these PKR KO macrophages several times for independent experiments. Batch-to-batch consistency has been good, and the provided QC data matched our in-house validation.
Helpful technical support and easy handling
Our team was initially concerned about handling primary-like macrophages, but the thawing and culture instructions were very straightforward. When we had questions about multiplicity of infection and timing for antiviral readouts, the technical support team responded quickly with practical suggestions.
Strong viability and good morphology
The shipment arrived on dry ice in excellent condition, and viability exceeded what we expected. After seeding, the cells showed typical macrophage morphology and remained healthy throughout the experiment. We were able to perform cytokine profiling and gene expression analysis without issues.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.