Macrophages are specialized immune cells that play a crucial role in the body's defense against pathogens and help maintain tissue homeostasis. They are a diverse group of cells with distinct functions and developmental pathways. Tissue-resident macrophages are found throughout the body and are involved in a variety of biological processes such as immune response, tissue repair, and metabolism. Creative Biolabs offers a comprehensive tissue-specific macrophage development service, supported by our knowledgeable team of scientists, for a wide range of research applications.

Fig.1 Macrophages from specific tissues.^1,2

Tissue-specific Macrophage Development Service at Creative Biolabs

Macrophages play a vital role in maintaining tissue balance and responding to injuries and infections throughout the body's lifespan. Our service has the potential to isolate and culture the macrophages from specific tissues such as bone, lung, CNS, etc. To better evaluate these macrophages, we provide a wide range of specific markers. Incorporating advanced technologies, our seasoned scientists are willing to help global customers engineer or analyze the desired macrophages to meet your multiple demands. Based on our robust macrophage therapeutic platform, we have great confidence in answering each of you with ideal results in a timely manner. Our tissue-specific macrophage development service menu involves:

Microglia and CNS-associated Macrophage Analysis Service

Our service incorporates expertise in cell biology and advanced analysis technologies. We deliver a wide range of approaches such as immunostaining, flow cytometry, and molecular biology techniques. We also offer assays including electron microscopy analysis, reactivity analysis, neurotoxic factor release analysis, and pathological function analysis.

Bone-marrow Macrophage Analysis Service

Our service takes advantage of several advanced technologies such as real-time live cell imaging technology to evaluate morphology and surface markers to study bond-marrow macrophage differentiation, chemotaxis, phagocytosis, and efferocytosis, allowing for a better understanding of how bond-marrow macrophages function in health and disease.

Tissue-specific Macrophage Depletion Service

Our tissue-specific macrophage depletion service involves utilizing clodronate liposomes to effectively target and deplete macrophages in a specific tissue. The clodronate, enclosed in the liposomes, is ingested by macrophages and released gradually, leading to their destruction and induction of apoptosis.

Tissue-specific Macrophage Immortalization Service

Creative Biolabs' tissue-specific macrophage immortalization service utilizes two distinct mechanisms for immortalization. We are able to customize specific immortalization methods for macrophages from different tissues. We also have the ability to characterize these immortalized tissue-specific macrophages according to their specific markers and your intended applications.

Tissue-specific Macrophage Blocking Service

Tissue-specific macrophage blocking is a valuable experimental tool to dissect the contribution of macrophages to tissue biology, disease pathology, and treatment response. Creative Biolabs offers several methods for achieving tissue-specific macrophage blocking. These methods can be combined or customized to specific research questions and experimental requirements.

Single Cell Transcriptomics of Tissue-specific Macrophages

Our macrophage single-cell sequencing platform includes cell isolation and capture, microfluidic chip amplification, and data analysis. The platform solves two major challenges of single-cell sequencing: single-cell isolation and capture, and reverse transcription amplification of very low amounts of RNA.

Creative Biolabs delivers a series of macrophages derived from distinctive tissues and specific markers for better analysis and research on these macrophages.

Tab.1 Tissue-specific macrophage types & markers.

Related Products

In tissue-specific macrophage development services, we are dedicated to the research and development of macrophages with specific functions and characteristics to meet the needs of different biomedical research. In addition to this, we also offer specific products.

These products are carefully designed through our specialized development process, aiming to provide efficient solutions for researchers. We hope to provide you with comprehensive support to help your research run smoothly.

Service Features

Diverse Models

We are able to provide a variety of tissue-specific macrophage models for different research needs, including but not limited to liver, lung, adipose tissue and kidney-derived macrophages. We are able to adjust the polarization state of the cells according to the needs of our customers.

Combining Multiple Cell Types

We offer co-culture of macrophages with other immune cells in order to study cell-cell interactions and their combined effects on immune responses. Our technology supports the long-term culture of macrophages, maintaining their functionality and phenotypic stability.

Functional Assessment

We can provide detailed functional analysis, including cell activity, phagocytosis, cytokine secretion analysis, as well as immune response after induction, to help our clients to deeply understand cell properties.

Fast Delivery

We provide detailed experimental records and data reports, and are committed to improving efficiency and shortening research cycles to ensure delivery of high-quality macrophage products within the agreed time.

Workflow of Tissue-specific Macrophage Development Services

Scientific Resources

Learn More Macrophage Biology

Download M1 vs. M2 Macrophages

Learn More Biological Characterization of Liver Macrophages

Learn More Isolation and Identification of Macrophages

Q & A

Q: How do you ensure that the developed macrophages are tissue-specific?

A: We accurately identify and isolate macrophage subpopulations in target tissues through single-cell suspension preparation of tissue samples and specific marker screening techniques, combined with flow cytometric sorting and gene expression analysis. For example, model cells such as THP-1 or RAW264.7 were utilized for functional validation to ensure cell specificity. In addition, cell phenotypic changes are dynamically tracked through bioinformatics analysis to ensure the stability of specific features during development.

Q: What are some key factors to consider when selecting tissue-specific macrophages?

A: There are several key factors you need to consider when selecting tissue-specific macrophages, including the biology of the target tissue, the desired cell phenotype and function, the specific needs of the experimental design, and the availability of the cells. Our team will help you analyze these factors to make the best choice.

Q: Do you have samples available for me to do preliminary testing?

A: We usually provide small samples for initial testing so that you can evaluate the performance and suitability of our products before deciding to go for large scale development. Please contact our team for more details.

Q: How do I place an order and is the order process complicated?

A: The process of placing an order is very simple. All you need to do is to contact our customer service team with your requirements and relevant information, and we will provide you with a detailed quotation and proposal. The whole process is efficient and transparent, so you don't have to worry about tedious steps.