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Human PB CD14+ Monocytes (Age: 23), 5 x 10^7 cells (MTS-1022-JF10)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can you support macrophage differentiation at scale for screening or repeated assay runs?

Yes. We can provide optional support for differentiation and polarization at defined timepoints, including standardized phenotyping and functional endpoints. For screening pipelines, clients often want reproducible macrophage states. We can align on your assay acceptance criteria and deliver a consistent "cells + QC + data" package that reduces variability and saves internal labor, particularly when your team is balancing many parallel projects.

What are common pitfalls when moving from monocytes to macrophages, and how can we avoid them?

The most common pitfalls are inconsistent density, variable adherence windows, and stimulation timing that doesn't match macrophage maturation. We recommend a controlled rest period after thaw, consistent plate coating decisions (if used), and clear day-by-day scheduling for medium changes and cytokine additions. For polarization, avoid "one-size-fits-all" timepoints-optimize based on your readouts (surface markers vs cytokines vs gene expression). We provide practical troubleshooting support and can recommend an efficient optimization matrix to reduce trial-and-error.

How do you ensure cells arrive in usable condition for time-sensitive studies?

We use controlled cryoshipping and clear receipt instructions to support immediate transfer to long-term storage. For time-sensitive programs, we encourage scheduling delivery so the lab is ready for transfer and thaw. If you need coordination around weekends/holidays or specific receiving windows, we can plan logistics to reduce risk. We also support rapid troubleshooting if any handling or performance questions arise upon receipt.

This product is a practical, high-performing option
We often see lot-to-lot variability with primary monocytes, but this vial was remarkably consistent. Thawing was straightforward, recovery was strong, and we could seed immediately into differentiation without losing too many cells. In our macrophage assays, we got stable, reproducible readouts for phagocytosis and inflammatory mediator production.
We will consider ordering additional age-matched donors to expand our cohort
The cells showed low spontaneous activation, which made our baseline controls stable. They also differentiated cleanly into macrophages with consistent morphology and robust phagocytic capacity. We appreciated the practical handling notes-especially guidance to minimize clumping and reduce stress during washing.
Our lab runs high-throughput macrophage polarization studies, and consistency is everything
These monocytes were easy to handle and integrated seamlessly into our standardized pipeline. We saw good viability post-thaw, and the cells tolerated medium changes without the dramatic losses we sometimes encounter. The macrophages derived from this prep responded strongly to classical and alternative polarization cues, giving us crisp contrasts in surface markers and transcript levels.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.