Online Inquiry

Human PB CD14+ Monocytes (Age: 65), 2.5-5 x 10^7 cells (MTS-1022-JF54)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

This listing shows 2.5×10^7 and 5×10^7 cells. How should I choose the right size for my study?

Choose 2.5×10^7 when you're running a focused assay panel or a single differentiation workflow; choose 5×10^7 for multi-condition screening, multiple readouts (flow + RNA + secreted factors), or heavier replication. Keeping size aligned with your plate format and time points reduces waste and helps maintain consistency.

Is the product still flow-characterized if I order the 2.5×10^7 option?

Yes.The lot is described as characterized by flow cytometry to ensure enrichment of CD14+ cells, supporting confidence in the starting population regardless of the selected vial size.

Can I re-freeze leftover cells if I only use part of the vial?

We do not recommend it. The product guidance explicitly advises avoiding repeated freezing and thawing, as this can reduce viability and alter functional phenotypes-particularly important for monocytes that respond quickly to stress signals. Plan to use the vial fully after thaw.

The vial size was flexible for our experimental design, and recovery was strong after thaw
We obtained consistent differentiation with M-CSF and saw clear polarization responses in our endpoint flow markers. The product helped us maintain schedule discipline because we could plan plate layouts accurately without relying on unpredictable fresh isolations.
Support provided practical advice for minimizing variability in culture conditions
The monocytes handled well, and we were able to proceed through differentiation without signs of excessive stress. Background contamination appeared low, and our gene expression profiles were more interpretable compared with mixed preparations. The donor age information was clearly communicated, which was important for our study design.
Our team integrated these monocytes into a microplate automation workflow
The cells tolerated standardized thaw/wash steps and maintained consistent seeding behavior, which is critical for automation. Differentiation proceeded predictably, and we were able to compare multiple conditions without worrying that the starting population was drifting.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.