Products for Macrophage Engineering

Lentiviral vectors are powerful tools for gene delivery in various applications. They are particularly effective for transfecting macrophage cell lines. Here's a product list of the delivery systems using lentiviral vectors specifically for infectious transfection into macrophages, intended for research use.

You can check out the detailed product catalog below.

Product Overview

Our products can efficiently transduce both dividing and non-dividing cells, including primary macrophages and established macrophage cell lines. Lentiviral vectors can be delivered to macrophage cell lines through several methods, optimizing transduction efficiency.

We offer various products that incorporate lentiviral vectors for macrophage transfection. These might include:

• Pre-packaged Lentiviral Vectors: Ready-to-use solutions containing the vector of interest for immediate application in macrophage cell lines.
• Custom Vector Services: Options for researchers to design lentiviral vectors tailored to their specific research requirements.
• Accessory Products: Including transduction enhancers, reagents for cell culture, and safety kits for monitoring the transduction processes.

Related Services

In addition to providing lentiviral manufacturing services characterized by strict quality control to deliver high-quality lentiviral products to our customers worldwide, we also offer in vivo lentivirus-based macrophage reprogramming services.

You can browse below and click to learn more.

Publications

Hanxu Zeng et al. investigated the regulatory role of TAB1 in glycolysis and macrophage activation during diabetic nephropathy (DN). They used siRNA to inhibit TAB1 in high glucose (HG)-stimulated bone marrow-derived macrophages and lentiviral vector-mediated knockdown of TAB1 in diabetic mice, and then assessed the modulatory effects on macrophage activation.

Results of studies using lentiviral vector technology to knock down TAB1 in diabetic mice and assess their glycolysis, macrophage polarization, and inflammation levels showed that glucose dysregulation initiates macrophage glycolysis and M1 polarization, causing the release of inflammatory factors. Down-regulation of TAB1 signaling reverses macrophage glycolysis and M1 polarization and attenuates inflammation.

Fig. 1 Effects of TAB1 LV on the expression of TAB1, downstream signaling pathway molecules and infiltration of macrophages.¹

Applications

We provide reliable tools for transducing macrophage cell lines to help researchers advance macrophage research.

• Gene Overexpression or Knockdown Studies
  
  • Our products are ideal for introducing genes to study their effects on macrophage differentiation, polarization and immune function.
  • Vector-delivered RNAi or CRISPR/Cas9 systems can knockout or knockdown target genes to help researchers study the function of genes in immune signaling pathways, phagocytosis, or cytokine production.
• CRISPR/Cas9-mediated Genome Editing
  Our products are capable of stably expressing Cas9 and directing RNA for genome editing, enabling precise gene modification in macrophages.

Scientific Resources

Q & A

Q: Can I use lentiviral vectors for stable transfection in primary macrophages?
A: Absolutely! Lentiviral vectors are particularly effective for stable transfection in primary macrophages, which often prove difficult to transfect with conventional methods. The ability of lentiviral vectors to integrate into the host genome allows you to achieve long-term expression of the transgene in these primary cells. Just ensure to select a compatible viral titer and optimum MOI for your specific macrophage preparation for the best results.

Q: What are the storage conditions for lentiviral vectors to ensure their stability?
A: Lentiviral vectors should be stored at -80°C for long-term stability. For short-term use, they can be kept at -20°C for a few weeks, but frequent freeze-thaw cycles should be avoided as they can degrade the viral particles. Additionally, it's advisable to aliquot lentiviral stocks into smaller volumes before freezing to limit exposure each time a portion is used.

Q: Do you provide technical support or guidance for using vectors in macrophage research?
A: Yes, we offer comprehensive technical support for researchers using our vectors. Our expert team can assist you with troubleshooting transfection issues, optimizing your protocols, and selecting the right vectors for your specific research needs. Whether you need guidance on experimental design, transduction efficiency, or safety practices, we are here to help you achieve your research goals. Don't hesitate to reach out for personalized support!

Q: How can I measure transduction efficiency in my macrophage cell line?
A: Transduction efficiency can be assessed using fluorescence microscopy, flow cytometry, or qPCR, depending on the reporter gene included in the vector. For macrophages, GFP or RFP reporter genes are commonly used for easy visualization. If you have specific assay preferences, our technical team can provide guidance on compatible measurement techniques and support for maximizing transduction outcomes.

Customer Reviews

Reference

1. Zeng, Hanxu, et al. "TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy." Inflammation Research 69 (2020): 1215-1234, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/).