Macrophage Marker Development Service
Our Service Related Products Service Features Workflow Scientific Resources Q & A
Rapid identification of macrophages and their polarization states provides valuable information for drug discovery, toxicological screening, and immunotherapy evaluation. Equipped with a team of skilled scientists with an innovative platform, which is designed specifically to meet very challenging requirements in macrophage development, Creative Biolabs offers fast, reliable support for our clients' macrophage marker development projects.
Macrophage Marker
Macrophages play a crucial role in orchestrating immune responses against pathogens and foreign materials. In response to environmental cues, macrophages have remarkable plasticity and perform a wide range of immune- and homeostasis-related functions. Due to their plasticity, macrophages can polarize into a spectrum of activated phenotypes. The two extremes in the spectrum are represented by the pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes.
Fig.1 Macrophage polarization and specific functions of M1 and M2 macrophages.1, 2
M1 macrophages are related to inflammation, including secretion of pro-inflammatory cytokines, engulfment of foreign entities, generation of reactive oxygen and nitrogen species, and assistance in T-helper type1 (Th1) cell responses to fight infection. M2 macrophages perform anti-inflammatory and wound repair functions.
The complexity associated with macrophage activation limits the ability of current biomarker-based methods to rapidly identify unique activation states. Given the significance and complexity of the roles macrophages play in biology and disease, it is necessary to develop markers that distinguish macrophages from other cells or identify their polarization state.
Macrophage Marker Development Services at Creative Biolabs
Macrophages engulf and digest microbes, cellular debris, and various disease-associated cells throughout the body. To characterize macrophages comprehensively, the key is using reliable Common Markers to distinguish macrophages from other cell types. For the two extremes M1 and M2 of macrophages, quantification of cell surface markers, transcription factors and cytokine profiles are usually used to distinguish between them. However, the complexity of the process of their activation limits the usage of current markers. Therefore, more novel M1/M2 macrophage markers are needed.
Macrophage Common Marker Development Service
A team of outstanding scientists at Creative Biolabs has focused on the discovery and development of common markers of macrophage for many years. Our technology is mainly based on transcriptional profiling analysis by bulk RNAseq/single-cell RNAseq and qPCR. Macrophage-specific genes can be identified effectively through this strategy and some auxiliary methods.
M1/M2 Macrophage Marker Development Service
The markers we identified are M1-exclusive or M2-exclusive, and they are further confirmed by flow cytometry. After that, RT-PCR is usually used to examine a panel of transcripts to verify the reproducibility of the gene expression changes. The markers identified could be used to distinguish between M1 and M2 macrophage, and better characterize TAM as well as other macrophage populations collected from humans and mice.
Creative Biolabs has developed several transcriptome-based approaches, including RNAseq, single-cell RNAseq, and microarrays, for the identification of novel macrophage markers. Macrophage-specific markers and M1/M2 macrophage-specific markers can be identified effectively through this high-throughput system. Moreover, to increase the accuracy of the results, some auxiliary methods, such as subcellular localization analysis, immune function analysis and immunohistochemistry assay, are included.
Professional technical scientists and abundant experience make Creative Biolabs a perfect partner to help our clients with the development of new macrophage markers. Through our high-throughput system, clients could accelerate their research with the most comprehensive view of macrophages and their polarization. For more detailed information, please feel free to contact us or send us your inquiry or question.
Related Products
In the field of macrophage research, the development of markers is crucial. We not only provide high-quality macrophage marker development services, but also introduce a series of related products. These products include cells, assay kits and cell culture media, which are designed to help researchers conduct macrophage-related experiments and analysis more efficiently.
Our products are based on advanced technology platforms that ensure reliability and accuracy, providing solid support for your research. Whether you need customized development or are looking for an off-the-shelf solution, our team will provide you with a full range of services and support.
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Cat.No
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Product Name
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Product Type
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MTS-0922-JF10
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Human Macrophages, Alveolar
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>Human Macrophages
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MTS-0922-JF99
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Human M0 Macrophages, 1.5 x 10^6
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>Human M0 Macrophages
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MTS-0922-JF52
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C57/129 Mouse Macrophages, Bone Marrow
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>C57/129 Mouse Macrophages
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MTS-0922-JF7
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Human M2 Macrophages, Peripheral Blood, 10 x 10^6
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>Human M2 Macrophages
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MTS-0922-JF34
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CD1 Mouse Macrophages
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>CD1 Mouse Macrophages
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MTS-1123-HM6
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Macrophage Colony Stimulating Factor (MCSF) ELISA Kit, Colorimetric
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>Detection Kit
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MTS-1123-HM15
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Macrophage Chemokine Ligand 19 (CCL19) ELISA Kit, qPCR
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>Detection Kit
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MTS-1123-HM17
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Macrophage Chemokine Ligand 4 (CCL4) ELISA Kit, Colorimetric
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>Detection Kit
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MTS-1123-HM49
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Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, Colorimetric
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>Detection Kit
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MTS-1123-HM42
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Macrophage Receptor with Collagenous Structure ELISA Kit, Colorimetric
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>Detection Kit
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Service Features
Professional Technical Support
We have a team of experienced scientists and technologists specializing in macrophage-related research. Our team is able to provide customized technical support, including experimental design, data analysis and interpretation of results, to ensure that our clients' research goals are achieved.
High Sensitivity and Specificity
Our macrophage marker development utilizes the latest technologies and methodologies to ensure that the markers developed are highly sensitive and specific. This means that we are able to recognize and differentiate between different types of macrophages, increasing the accuracy and reliability of our results and thus advancing research.
Rapid Response & Flexibility
We are committed to providing our clients with rapid service response and flexible solutions. Whether it is the scope, scale or time requirements of the project, we are able to adjust to the needs of our customers to ensure the efficiency and timeliness of our services.
Diverse Application Scenarios
Our services can be used in a wide range of applications from basic research to therapy development. Whether it is in cancer, inflammatory diseases or immune-related research, we can provide our clients with effective solutions to drive innovation and progress in their research.
Workflow of Macrophage Marker Development Services
Requirements Analysis and Design of Experiments Sample Preparation Marker Screening Validation Experiment Data Analysis and Interpretation Report Writing and Feedback
Scientific Resources
Q & A
Q: What modeling systems are used in screening experiments for macrophage markers?
A: We usually use the following model systems in our macrophage marker screening experiments:
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Primary cells: we can extract primary macrophages from human or mouse tissues for studies under realistic physiological conditions.
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Cell lines: macrophage cell lines that are frequently used in immunological studies, such as THP-1 or RAW264.7 cells, are utilized to ensure reproducibility and standardization of experiments.
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In vivo models: such as mouse models to assess the expression and function of macrophage markers in physiological and pathological states.
Q: What control experiments do you include in your validation experiments for macrophage markers?
A: In the validation experiments for macrophage markers, we perform the following types of control experiments:
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Negative control: use cells or samples that do not express the target marker to confirm the specificity of the experiment.
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Positive control: cells or samples known to express the marker are used to ensure the validity of the assay.
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Technical repeat: multiple repetitions of the same experiment are performed to verify the reproducibility and consistency of the results.
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Sampling at different time points: samples are collected at different time points and conditions to assess dynamic changes.
Q: Can your services meet the macrophage marker development needs of different species?
A: Absolutely! We have extensive experience developing macrophage markers for a wide range of animal models and human samples. Whether it's mice, rats, or other species, our team can provide customized solutions for specific needs.
Q: How are your service fees calculated?
A: The cost of our services is usually assessed based on factors such as the complexity of the project, time and resources required. After initial communication, we will provide our clients with a detailed quote and description of our services so that they can clearly understand the components of each fee.
