Macrophages and regulatory T cells are essential components of the immune system. By releasing different cytokines, they interact and regulate each other to avoid an overactive immune response. This crucial process helps maintain the body's immune equilibrium. To deeply understand the interaction mechanism between them, Creative Biolabs specifically delivers a macrophage-regulatory T cell interaction analysis service. Leveraging our advanced macrophage therapeutic platform, our experienced team of scientists collaborates with you to devise comprehensive solutions that cater to your specific needs in the realm of macrophage-regulatory T cell interactions.

Interaction Mechanism Between Macrophages and Regulatory-T Cells

• The exchange of signaling molecules: Macrophages and regulatory T cells have the ability to mutually exchange signaling molecules, facilitating the transmission of information. These signaling molecules include cytokines, chemokines, or other chemical messengers.
• Surface receptor interaction: Both macrophages and regulatory T cells possess a multitude of surface receptors, including TLR and CD40, enabling mutual recognition.
• Antigen presentation: Macrophages, functioning as antigen-presenting cells, are capable of engulfing and processing antigens before presenting them to regulatory T cells.
• Cell contact-dependent interactions: Macrophages and regulatory T cells can engage in direct cell-cell contact, which further modulates the magnitude and orientation of the immune response.

Fig.1 Tregs interact with other cells in acute lung injury.¹

Macrophage-Regulatory T Cell Interaction Analysis Service at Creative Biolabs

Macrophage-regulatory T cell interaction analysis service from Creative Biolabs incorporates various advanced technologies to achieve rapid and effective interaction analysis results presentation.

We deliver a series of approaches that can be identified as highly sensitive and high throughput to design for customers' projects. Through utilizing these approaches, customers are able to comprehensively analyze the interaction between macrophages and regulatory T cells from various dimensions such as cytokine expression levels, surface receptors, regulatory relationships among specific genes, etc. The application of distinct analytical metrics necessitates the utilization of specific analytical methodologies. Our seasoned scientists also provide custom solutions to solve any obstacles throughout the projects. At Creative Biolabs, we will try our best to help our customers obtain the desirable results to develop more macrophage therapeutics.

• Co-culturing of macrophages and regulatory T cells.
• The detection of surface receptors by flow cytometry.
• The detection and identification of cytokines.
• Transcriptome analysis.
• Chromatin immunoprecipitation (ChIP).
• Immunofluorescence staining.

Publication

In this study, researchers investigated the role of macrophage intrinsic RELMα in suppressing proinflammatory T-cell responses. They co-cultured peritoneal macrophages with spleen cells in vitro and found that RELMα-expressing macrophages stimulated significant proliferation of regulatory T cells (Tregs), which are important for suppressing inflammation. These findings suggest a novel regulatory pathway involving RELMα that helps limit type 2 inflammation.

Fig.2 Macrophage-derived RELMα enhances the proliferation of regulatory T cells.²

If you are interested in our macrophage-regulatory T cell interaction analysis service, please don't hesitate to get in touch with us for more details.