KG-1 cells are commonly used in research as a model system for studying myeloid differentiation, leukemia biology, and the effects of various compounds on myeloid cell growth and differentiation. KG-1 cells have been particularly useful in investigations related to hematopoiesis, leukemia pathogenesis, and drug screening studies. Creative Biolabs develops a macrophage model based on KG-1 cells, which consists of culturing and inducing differentiation into macrophage-like cells.

KG-1 Cells

KG-1 cells are a human myeloblastic leukemia cell line derived from the peripheral blood of a patient with acute myelogenous leukemia (AML). KG-1 cells are characterized by their ability to differentiate into mature myeloid cells, including granulocytes, monocytes, and macrophages, under appropriate culture conditions.

Key characteristics of KG-1 cells include their suspension growth in culture, relatively low proliferation rate, and responsiveness to differentiation-inducing agents such as phorbol esters (e.g., phorbol 12-myristate 13-acetate, PMA) and vitamin D3. They are often utilized as a tool for understanding the mechanisms underlying myeloid differentiation and for screening potential therapies targeting leukemia or myeloid disorders.

Fig.1 KG-1 cells in culture flask.¹

KG-1-based Macrophage Model Development Service at Creative Biolabs

Creative Biolabs has expertise in cell culture, differentiation protocols, immunological assays, and data analysis. When providing macrophage model development services for KG-1, models can also be customized to meet your specific research needs and goals.

Frequently Asked Questions

Q1: Why choose the KG-1-based macrophage model?

A1: Choosing a KG-1-based macrophage model over other options like HL-60 or THP-1 cells depends on the specific research needs and goals.

• KG-1 cells are derived from a patient with acute myelogenous leukemia (AML) and possess monocytic characteristics. This monocytic lineage may make them particularly suitable for modeling certain aspects of macrophage biology, as they are closer to the monocyte/macrophage lineage than other cell lines like HL-60.
• KG-1 cells have been shown to differentiate into macrophage-like cells in response to various differentiation agents. This differentiation potential can be harnessed to generate a macrophage model that closely resembles primary macrophages.
• Since KG-1 cells are derived from a leukemia patient, they may retain certain disease-related characteristics or responses that could be relevant for studying diseases involving macrophages, such as leukemia-associated macrophage biology or interactions between leukemia cells and the microenvironment.
• KG-1 cells can be manipulated genetically or treated with specific compounds to study various aspects of macrophage biology.

Creative Biolabs has always put the needs of our customers first, and if you have any questions about the KG-1 macrophage model, you can contact us directly for assistance.