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Human M1 Macrophages, Peripheral Blood (Age: 30), 5 x 10^6 (MTS-0922-JF9)

Datasheet

Overview

Description

This product is isolated from human peripheral blood. Donors are screened for hepatitis B, hepatitis C, HIV-1 and HIV-2. 5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Human

Product Type

Human M1 Macrophages

Specification

Cell Source

Peripheral blood

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these cells ready to use immediately after thawing?

Yes. After thawing and a brief recovery period in the recommended culture medium (to allow them to adhere), they are ready for your experiment. They are shipped as a fully differentiated and polarized M1 population.

What is the recommended cell plating density?

The optimal plating density depends on your specific assay (e.g., 96-well vs. 6-well plate) and application (e.g., cytokine analysis vs. imaging). A detailed protocol with recommended densities will be provided with your purchase.

Can I re-freeze these cells if I don't use the whole vial?

We strongly advise against refreezing. These are primary cells, and an additional freeze-thaw cycle will severely impact their viability and functional integrity.

Will order this specific lot again
The 5 million count is generous and allows for multiple experiments. We verified their M1 phenotype with flow and ELISA, and they were spot-on. A very reliable product.
Creative Biolabs has been a good supplier for us
Used these for a drug screening assay. The cells were active and gave us a strong, reproducible signal. It saves us so much time not having to isolate and polarize PBMCs ourselves.
We specifically needed M1 macrophages from a donor in their 30s
I'm very impressed with these cells. They arrived on Monday, and I had my experiment running by Tuesday. The viability was fantastic. The pro-inflammatory cytokine profile was textbook M1.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.