Online Inquiry

Rat Macrophages, Spleen, 5 x 10^5 (MTS-0922-JF97)

Datasheet

Overview

Description

This product is isolated from the spleen of pathogen-free laboratory rat. 5 x 10^5 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Rat

Product Type

Rat Macrophages

Specification

Cell Source

Spleen

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

5 x 10^5 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How are the splenic macrophages isolated and enriched to ensure purity?

The spleen tissue is gently dissociated using enzymatic and mechanical methods, followed by immunomagnetic enrichment or other selective techniques to isolate macrophage populations while minimizing contamination by lymphocytes and other cell types. Each lot is checked for sterility, mycoplasma, and macrophage marker expression.

Are these cells compatible with flow cytometry, and do they tolerate staining and sorting?

Yes. The cells retain stable expression of macrophage and activation markers and can be labeled with fluorochrome-conjugated antibodies for surface and intracellular staining. They generally tolerate flow cytometric analysis well. When cell sorting is required, we recommend using gentle sorting parameters and promptly re-plating the cells to allow them to recover, especially if you plan to perform downstream functional assays.

What storage and handling conditions do you recommend for long-term use of this product?

For long-term storage, vials should be kept in the vapor phase of liquid nitrogen. We do not recommend extended storage at -80°C beyond the transport period. Once thawed, cells should be used immediately; refreezing is not advised, as it may significantly impair viability and function. Following our thawing protocol and avoiding unnecessary temperature fluctuations will help preserve the full functional capacity of the cells.

We used these splenic macrophages for antigen-presentation and T-cell co-culture experiments
The cells showed strong MHC II expression and produced robust cytokine responses. Our T-cell activation readouts were very clear, and the model closely matched our expectations from in vivo data.
The enrichment and purity were clearly better than our in-house isolations
For our host-pathogen work, these macrophages provided a reliable platform to study uptake and intracellular responses. They handled bacterial exposure well and gave reproducible cytokine and gene-expression profiles across multiple experiments.
Overall, a very solid product
The macrophages were responsive, easy to handle, and compatible with both imaging and plate-based assays. The combination of phagocytic function and antigen-presenting capacity made them a versatile tool in our immunology projects.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.