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Rat Macrophages (with GFP expression), Bone Marrow, 0.5 x 10^6 (MTS-0922-JF87)

Datasheet

Overview

Description

With GFP expression, this product is isolated from the bone marrow of pathogen-free laboratory rat. 0.5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Rat

Product Type

Rat Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How stable is GFP expression over the course of typical experiments, and does it affect macrophage function?

The GFP transgene is stably integrated, and each batch is verified by flow cytometry to ensure a high percentage of GFP‑positive cells. Under standard culture conditions and within the usual experimental window of several days, GFP expression remains bright and uniform.

What imaging platforms are compatible with these GFP-expressing macrophages?

These macrophages are compatible with standard fluorescence microscopes, confocal imaging systems, and high‑content screening platforms. They adhere well to plastic and glass, which supports both fixed and live‑cell imaging. The GFP signal is bright enough for time‑lapse studies and multiplex experiments, provided that fluorophores are chosen to minimize spectral overlap.

Can I combine GFP macrophages with fluorescent pathogens or particles to study phagocytosis and trafficking?

Yes. A common application is dual‑color imaging in which GFP labels the macrophages and a second fluorophore labels pathogens, apoptotic cells, or synthetic particles. This setup allows you to visualize binding, uptake, and intracellular trafficking in real time or in fixed preparations.

Operationally, the cells were easy to handle
They thawed well, attached robustly to our imaging plates, and kept strong GFP expression for the duration of our 3-4 day experiments. Quantification of cell behavior using automated image analysis was straightforward, which greatly improved throughput.
We used GFP macrophages together with fluorescent bacteria to study phagocytosis and intracellular trafficking
The two‑color imaging produced clear and striking visuals that were extremely helpful for presentations and manuscripts. Having a ready‑to‑use GFP macrophage product significantly accelerated our assay development.
The product quality and responsive technical support made the transition very smooth, and we now rely on these cells for several ongoing projects.
The availability of off‑the‑shelf GFP‑labeled macrophages allowed us to prototype new imaging‑based assays without the time and uncertainty of generating our own stable lines.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.