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BALB/C Mouse Macrophages, Bone Marrow, 6 x 10^6 (MTS-0922-JF27)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory BALB/c mouse. 6 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

BALB/C Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

6 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do these BALB/c BMDMs differ from your C57BL/6 (B6) BMDMs?

While both are M0 macrophages, their genetic background is the key difference. BALB/c macrophages are known to polarize more readily to an M2 (anti-inflammatory) phenotype and produce a different cytokine profile upon stimulation compared to B6 macrophages, which are Th1-biased.

What is the main research application for BALB/c strain BMDMs?

The BALB/c mouse strain is known for its strong Th2-biased immune response. Therefore, these BALB/c-derived BMDMs are the ideal choice for in vitro studies related to asthma, allergic inflammation, parasitic infections, and other Th2-mediated diseases.

What is the cell count and phenotype?

This vial contains 6 x 10^6 (6 million) cells. They are differentiated into naïve (M0) macrophages from bone marrow precursors, making them a perfect blank slate for your polarization experiments.

Saved us the time and effort of harvesting from our own BALB/c colony
They polarize to M2 with IL-4 stimulation beautifully and give us very clean, reproducible data. High viability.
Good, clean BMDMs
We bought both these BALB/c and the C57BL/6 BMDMs to compare their response to our compound. The strain difference was clear! Great quality cells, and the 6M count is generous.
AUsed these for a Leishmania infection assay
Solid product. Adhered well and looked healthy. The protocol was straightforward. A bit pricey, but the convenience and quality are worth it.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.